Splicing of exon 9a in FMR1 transcripts results in a truncated FMRP with altered subcellular distribution

•We identified a new 46 bp microexon terming the exon 9a.•Inclusion of exon 9a introduces an in-frame termination codon.•Exon 9a-containing transcripts could produce a 34 kDa truncated protein.•Truncated protein displayed both nuclear and cytoplasmic localization. FMRP is an RNA-binding protein, los...

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Bibliographic Details
Published in:Gene 2020-03, Vol.731, p.144359-144359, Article 144359
Main Authors: Fu, Xian-guo, Yan, Ai-zhen, Xu, Yong-jun, Liao, Juan, Guo, Xiao-yan, Zhang, Duo, Yang, Wen-jing, Zheng, De-zhu, Lan, Feng-hua
Format: Article
Language:English
Subjects:
Adult
Alternative splicing
Alternative Splicing - physiology
Case-Control Studies
Cell Nucleus - metabolism
Cytoplasm - metabolism
Exon 9a
Exons - genetics
FMR1
FMRP
Fragile X Mental Retardation Protein - genetics
Fragile X Mental Retardation Protein - metabolism
Fragile X syndrome
Fragile X Syndrome - genetics
Fragile X Syndrome - metabolism
Fragile X Syndrome - pathology
HEK293 Cells
HeLa Cells
Humans
Male
Protein Isoforms - genetics
Protein Isoforms - metabolism
RNA Splicing - physiology
Tissue Distribution
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Summary:•We identified a new 46 bp microexon terming the exon 9a.•Inclusion of exon 9a introduces an in-frame termination codon.•Exon 9a-containing transcripts could produce a 34 kDa truncated protein.•Truncated protein displayed both nuclear and cytoplasmic localization. FMRP is an RNA-binding protein, loss of which causes fragile X syndrome (FXS). FMRP has several isoforms resulted from alternative splicing (AS) of fragile X mental retardation 1 (FMR1) gene, but their biological functions are still poorly understood. In the analysis of alternatively spliced FMR1 transcripts in the blood cells from a patient with FXS-like phenotypes (normal CGG repeats and no mutation in coding sequence of FMR1), we identified three novel FMR1 transcripts that include a previously unidentified microexon (46 bp), terming the exon 9a. This microexon exists widely in unaffected individuals, inclusion of which introduces an in-frame termination codon. To address whether these exon 9a-containing transcripts could produce protein by evading nonsense-mediated decay (NMD), Western blot was used to analysis blood cell lysate from unaffected individuals and a 34 kDa protein that consistent in size with the molecular weight of the predicted truncated protein produced from mRNA with this microexon was found. Meanwhile, treatment of peripheral blood mononuclear cells with an inhibitor of NMD (Cycloheximide) did not result in significant increase in exon 9a-containing transcripts. Using confocal immunofluorescence, we found the truncated protein displayed both nuclear and cytoplasmic localization in HEK293T and HeLa cells due to lacking C-terminal domains including KH2, NES, and RGG, while the full-length FMRP protein mainly localized in the cytoplasm. Therefore, we hypothesize that the inclusion of this microexon to generate exon 9a-containing transcripts may regulate the normal functionality of FMRP, and the dysregulation of normal FMRP due to increased exon 9a-containing alternatively spliced transcripts in that patient may be associated with the manifestation of FXS phenotype.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2020.144359