RANK/RANKL/OPG gene polymorphisms and loss of orthodontic mini‐implants

Objective To investigate the association of genetic polymorphisms (tagSNPs type) of RANK/RANKL/OPG genes with the loss of orthodontic mini‐implants (MIs). Setting and sample population One hundred and thirty‐five patients of both sexes, with mean age of 48.7 ± 10 (20‐76 years), were studied. The con...

Full description

Saved in:
Bibliographic Details
Published in:Orthodontics & craniofacial research 2020-05, Vol.23 (2), p.210-222
Main Authors: Giacobbo, Laís Cristina, Perin, Maria Augusta Andrigo, Pereira, Thaís Munhoz, Garmendia, Mariana Oliveira, Reichow, Alexandre, Melo, Ana Cláudia, Castilhos, Bruno Borges, Trevilatto, Paula Cristina
Format: Article
Language:English
Subjects:
Acetic acid
Adult
Ammonium
Ammonium acetate
Buccal mucosa
Dental Implants
Dental Restoration Failure
Epithelial cells
Female
Gene polymorphism
genetic polymorphisms
Genotyping
Humans
Male
Middle Aged
mini‐implant
Mouthwashes
Multivariate analysis
OPG
Orthodontics
Osteoprotegerin
Polymerase chain reaction
Polymorphism
Polymorphism, Genetic
RANK
RANK Ligand
RANKL
Receptor Activator of Nuclear Factor-kappa B - genetics
TRANCE protein
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective To investigate the association of genetic polymorphisms (tagSNPs type) of RANK/RANKL/OPG genes with the loss of orthodontic mini‐implants (MIs). Setting and sample population One hundred and thirty‐five patients of both sexes, with mean age of 48.7 ± 10 (20‐76 years), were studied. The control group was composed of 104 patients, with no MI lost and functioning for at least 6 months and the case group, of 31 patients with at least one MI lost. Materials and methods Cells were obtained by mouthwash with 3% glucose solution for 1 minute and scraping the buccal mucosa with sterilized spatula. DNA was extracted from buccal epithelial cells with 10 M ammonium acetate and 1 mM EDTA. Genotyping was performed by the real‐time polymerase chain reaction (PCR) technique. Univariate and multivariate analyses were performed (P 
ISSN:1601-6335
1601-6343
DOI:10.1111/ocr.12360