Isolation and subunit compositions of the xylanosome complexes produced by Cellulosimicrobium species

•Xylanosomes produced by Cellulosimicrobium funkei and Cellulosimicrobium terreum were firstly isolated and functionally characterized.•Whole genome sequence of the two type strains were sequenced to fine scale.•Proteomic analysis of purified xylanosomes were performed using LC-MS/MS.•Subunit struct...

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Bibliographic Details
Published in:Enzyme and microbial technology 2020-02, Vol.133, p.109445-109445, Article 109445
Main Authors: Dou, Tong-Yi, Chen, Jing, Liu, Chenglin
Format: Article
Language:English
Subjects:
Actinobacteria - enzymology
Actinobacteria - genetics
Carbohydrate Metabolism
Cellulosimicrobium cellulans
Cellulosimicrobium funkei
Cellulosimicrobium terreum
Genus Cellulosimicrobium
Hemicellulose degradation
Multienzyme Complexes - chemistry
Multienzyme Complexes - genetics
Multienzyme Complexes - isolation & purification
Phylogeny
Proteomics
RNA, Ribosomal, 16S - genetics
Xylanosome
Xylans - metabolism
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Summary:•Xylanosomes produced by Cellulosimicrobium funkei and Cellulosimicrobium terreum were firstly isolated and functionally characterized.•Whole genome sequence of the two type strains were sequenced to fine scale.•Proteomic analysis of purified xylanosomes were performed using LC-MS/MS.•Subunit structure were revealed by the combination of genomic sequencing and proteomic results.•Comparative genomic analysis indicate diversities in their reponse to polysacchorides (herein corn cob) for energy. Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides. Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum). Functional studies and subunit structure identifications using genomic sequencing and proteomic techniques were also performed. When compared with the xylanosomes produced by C. cellulans F16, the isolated MECs showed a larger particle size and shared at least three conserved multidomain proteins. In addition, they also exhibited different enzymatic activities and subunit compositions, which indicates diverse capability and strategies in degrading hemicelluloses.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2019.109445