Genomic Regions Associated with Virulence in Pyrenophora teres f. teres Identified by Genome-Wide Association Analysis and Biparental Mapping

Net form net blotch (NFNB), caused by the fungal pathogen f. , is an important foliar disease present in all barley-producing regions of the world. This fungus is a hemibiotrophic and heterothallic ascomycete, where sexual recombination can lead to changes in disease expression in the host. Knowledg...

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Bibliographic Details
Published in:Phytopathology 2020-04, Vol.110 (4), p.881-891
Main Authors: Martin, Anke, Moolhuijzen, Paula, Tao, Yongfu, McIlroy, Judy, Ellwood, Simon R, Fowler, Ryan A, Platz, Greg J, Kilian, Andrzej, Snyman, Lisle
Format: Article
Language:English
Subjects:
Ascomycota
Australia
Genome-Wide Association Study
Genomics
Hordeum
Plant Diseases
Virulence
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Summary:Net form net blotch (NFNB), caused by the fungal pathogen f. , is an important foliar disease present in all barley-producing regions of the world. This fungus is a hemibiotrophic and heterothallic ascomycete, where sexual recombination can lead to changes in disease expression in the host. Knowledge of the genetic architecture and genes involved in virulence is vital to increase the durability of NFNB resistance in barley cultivars. We used a genome-wide association mapping approach to characterize f. genomic regions associated with virulence in Australian barley cultivars. One hundred eighty-eight f. isolates collected across five Australian states were genotyped using Diversity Arrays Technology sequence markers and phenotyped across 20 different barley genotypes. Association mapping identified 14 different genomic regions associated with virulence, with the majority located on f. chromosomes 3 and 5 and one each present on chromosomes 1, 6, and 9. Four of the regions identified were confirmed by quantitative trait loci (QTL) mapping. The QTL regions are discussed in the context of their genomic architecture together with examination of their gene contents, which identified 20 predicted effectors. The number of QTL shown in this study at the population level clearly illustrates a complex genetic basis of f. virulence compared with pure necrotrophs, such as the wheat pathogens and .
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO-10-19-0372-R