Development of sheet‐enhanced technique (Set) method for matrix‐assisted laser desorption/ionization imaging mass spectrometry

Rationale The key to successful experiments in matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI‐IMS) is to apply the matrix uniformly to the sample. With the development of automated equipment, uniform matrix application has made great progress while the sample preparatio...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 2020-04, Vol.34 (8), p.e8703-n/a
Main Authors: Nakashima, Yuko, Eto, Fumihiro, Ishihara, Kazuku, Yamazaki, Fumiyoshi, Sato, Shumpei, Sakurai, Takanobu, Kahyo, Tomoaki, Setou, Mitsutoshi
Format: Article
Language:English
Subjects:
Biomolecules
Desorption
Image acquisition
Ionization
Ions
Mass spectrometry
Scientific imaging
Spectroscopy
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Summary:Rationale The key to successful experiments in matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI‐IMS) is to apply the matrix uniformly to the sample. With the development of automated equipment, uniform matrix application has made great progress while the sample preparation required to acquire a better image becomes complicated. Methods The approach is to apply the matrix uniformly to tape and adhere it to the tissue section. We call this the sheet‐enhanced technique (Set) method. Results The Set method promotes ionization of biomolecules as well as the spray method. This procedure does not require the preparation and application of a matrix solution for each experiment, dramatically reducing the time and effort of matrix deposition. Conclusions In the present study, we have developed the Set method as a new matrix application method. The method promotes ionization of biomolecules as well as the spray method for MALDI‐IMS.
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.8703