Simple production of hydrophobin-fused domain III of dengue envelope protein and induction of neutralizing antibodies against the homotypic serotype of dengue virus

Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61....

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Bibliographic Details
Published in:Biotechnology letters 2020-03, Vol.42 (3), p.419-428
Main Authors: Cerezo, Julieta, Targovnik, Alexandra Marisa, Smith, María Emilia, González Maglio, Daniel, Luppo, Victoria Celina, Morales, María Alejandra, Miranda, María Victoria, Rodríguez Talou, Julián
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Applied Microbiology
Binary systems
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cross-reaction
Dengue fever
Fusion protein
Hydrophobins
Immunization
Larvae
Life Sciences
Microbiology
Neutralizing
Original Research Paper
Proteins
Serotypes
Vaccines
Vector-borne diseases
Viral diseases
Viral envelope proteins
Viruses
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Summary:Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusion protein DomIII serotype 2, represents an integrated strategy for simple production of recombinant antigens. Purified fusion proteins induced serotype-specific neutralizing antibodies without cross-reaction against other serotypes and arboviruses after mouse immunization. hydrophobin-fused domain III of dengue envelope protein could be a promising strategy for easy and low-cost production of components of a tetravalent sub-unit vaccine against dengue.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-019-02767-2