Intrafollicular lipopolysaccharide injection delays ovulation in cows

The aim of this study was to evaluate effects of intra-follicular (i.f.) treatment with lipopolysaccharide (LPS) on follicular and luteal development in cows. There were 18 non-lactating cows assigned to two groups to address this aim: control group (n = 9), which received an i.f. injection of salin...

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Published in:Animal reproduction science 2019-12, Vol.211, p.106226-106226, Article 106226
Main Authors: Gindri, Patrícia, de Ávila Castro, Natália, Mion, Bruna, Garziera Gasperin, Bernardo, Catarelli Pegoraro, Ligia Margareth, Alveiro Alvarado Rincón, Joao, Diniz Vieira, Arnaldo, Pradieé, Jorgea, Machado Pfeifer, Luiz Francisco, Nunes Corrêa, Marcio, Schneider, Augusto
Format: Article
Language:English
Subjects:
Animals
Bovine
Cattle
Female
Inflammation
Injections
Lipopolysaccharides - toxicity
LPS
Ovarian Follicle - drug effects
Ovary
Ovulation - drug effects
Progesterone - blood
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Summary:The aim of this study was to evaluate effects of intra-follicular (i.f.) treatment with lipopolysaccharide (LPS) on follicular and luteal development in cows. There were 18 non-lactating cows assigned to two groups to address this aim: control group (n = 9), which received an i.f. injection of saline; and LPS group (n = 9), which received an i.f. injection of 1 μg of LPS per mL of follicular fluid. Cows were treated with an intravaginal P4 releasing device (IVD) and estradiol benzoate on D0. On D4 and D5 cows were treated with cloprostenol sodium and on D7 the IVD was removed. At 12 h after IVD removal, cows were administered the i.f. injection of LPS or saline. After administration of these treatments, follicular development was evaluated every 12 h until ovulation. The LPS treatment increased blood flow in pre-ovulatory follicles (P = 0.05). Follicle growth was reduced by LPS injection (P < 0.02) resulting a longer period to the time of ovulation for cows in the LPS than control group (P = 0.03). The percentage of cows having ovulations was less for the LPS than control group (P = 0.03). The diameter of the CL, CL blood flow and P4 concentrations 5 and 12 days after ovulation did not differ between groups (P> 0.05). In conclusion, intra-follicular treatment with LPS resulted in a decreased rate of follicle growth, delayed timing of ovulations and a lesser number of cows having ovulations.
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2019.106226