Dissecting the Potential of Selenoproteins Extracted from Selenium-Enriched Rice on Physiological, Biochemical and Anti-Ageing Effects In Vivo

Ageing is an irreversible phenomenon and the processes which can delay it are under consideration for a long time by the scientific community. Selenium is an important candidate for it, but the impact of selenoprotein on nutritional changes and ageing has not been reported well. In this regard, anti...

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Bibliographic Details
Published in:Biological trace element research 2020-07, Vol.196 (1), p.119-130
Main Authors: Zeng, Rui, Farooq, Muhammad Umer, Zhang, Guo, Tang, Zhichen, Zheng, Tengda, Su, Yang, Hussain, Sajad, Liang, Yuanke, Ye, Xiaoying, Jia, Xiaomei, Zhu, Jianqing
Format: Article
Language:English
Subjects:
Administration, Oral
Ageing
Aging
Aging (artificial)
Aging - drug effects
Animals
Antioxidants
Antioxidants - administration & dosage
Antioxidants - chemistry
Antioxidants - pharmacology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Biphenyl Compounds - analysis
Biphenyl Compounds - antagonists & inhibitors
Components
D-Galactose
Diet
Enzymatic activity
Enzyme activity
Ethanol
Free radicals
Galactose
Galactose - administration & dosage
Gene expression
Genes
Glutathione
Glutathione peroxidase
Heme oxygenase (decyclizing)
Hepatocytes
Injections, Intraperitoneal
Injury prevention
Life Sciences
Liver
Male
Malondialdehyde
Mice
Mice, Inbred Strains
Nutrient content
Nutrition
Oncology
Oryza - chemistry
Peroxidase
Physiological effects
Picrates - analysis
Picrates - antagonists & inhibitors
Plant Extracts - administration & dosage
Plant Extracts - chemistry
Plant Extracts - pharmacology
Proteins
Scavenging
Selenium
Selenium - administration & dosage
Selenium - pharmacology
Selenoproteins
Selenoproteins - administration & dosage
Selenoproteins - chemistry
Serum
Superoxide dismutase
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Summary:Ageing is an irreversible phenomenon and the processes which can delay it are under consideration for a long time by the scientific community. Selenium is an important candidate for it, but the impact of selenoprotein on nutritional changes and ageing has not been reported well. In this regard, antioxidant activities and free radical scavenging effect of selenoproteins extracted from selenium-rich rice were studied. Mice were administered a subcutaneous abdominal injection of d -galactose to induce the ageing model and fed with different selenoprotein dosage diet. Deviations among biochemical activities (total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and malondialdehyde (MDA)) in liver and serum of the mice were assessed. The degree of liver injury, antioxidant genes and protein relative expression were estimated. The protein content, selenium content, hydroxyl scavenging and DPPH radicals were accessed in selenoprotein components. The selenoprotein constituent had protein and selenium contents in different components as water-soluble proteins > alkali-soluble proteins > salt-soluble proteins > ethanol-soluble proteins. The enzymatic activity (total antioxidant capacity, GSH-Px and SOD) in liver and serum of mice was significantly enhanced in selenoprotein diet groups. d -Galactose-induced liver injury was significantly reduced by selenoprotein diet of 25 μg/(kg day). Real-time qPCR and Western blot disclosed the enhanced relative expression of antioxidant genes ( SOD2 , GPX1 , TrxR2 and Nrf2 ) and HO-1 protein in the positive control (Vc) and selenoprotein diet groups. In conclusion, selenoprotein treatment was found to have a positive influence on liver hepatocytes and biochemical features in mice. It might be used as a potential diet in scavenging oxidative injury and supporting enzymatic antioxidant system.
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-019-01896-z