Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform

The rapidly growing mycobacterium Mycobacterium abscessus is a clinically important organism causing pulmonary and skin diseases. The M. abscessus complex is comprised of three subspecies: M. abscessus subsp. abscessus , M. abscessus subsp. massiliense , and M. abscessus subsp. bolletii . Here, we a...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2020-03, Vol.39 (3), p.551-558
Main Authors: Xiao, Guohui, Zhang, Su, Liang, Zhihang, Li, Guanqiang, Fang, Mutong, Liu, Yaya, Zhang, Juanjuan, Ou, Min, He, Xing, Zhang, Tianyu, Zeng, Changchun, Liu, Lei, Zhang, Guoliang
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Biomedical and Life Sciences
Biomedicine
CRISPR-Associated Proteins - genetics
CRISPR-Cas Systems
DNA, Bacterial
Endodeoxyribonucleases - genetics
Genes, Bacterial
Humans
Internal Medicine
Lung diseases
Medical Microbiology
Molecular Typing - methods
Mycobacterium abscessus
Mycobacterium abscessus - classification
Mycobacterium abscessus - genetics
Mycobacterium Infections, Nontuberculous - diagnosis
Mycobacterium Infections, Nontuberculous - microbiology
Nucleic acids
Original Article
Phylogeny
Polymerase Chain Reaction
RNA, Guide, CRISPR-Cas Systems
RpoB protein
Sequence Analysis, DNA
Skin diseases
Species
Workflow
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Summary:The rapidly growing mycobacterium Mycobacterium abscessus is a clinically important organism causing pulmonary and skin diseases. The M. abscessus complex is comprised of three subspecies: M. abscessus subsp. abscessus , M. abscessus subsp. massiliense , and M. abscessus subsp. bolletii . Here, we aimed to develop a Cas12a/sgRNA-based nucleic acid detection platform to identify M . abscessus species and subspecies. By designing specific sgRNA probes targeting rpoB and erm (41), we demonstrated that M. abscessus could be differentiated from other major mycobacterial species and identified at the subspecies level. Using this platform, a total of 38 clinical M. abscessus isolates were identified, 18 as M. abscessus subsp. abscessus and 20 as M. abscessus subsp. massiliense . We concluded that the Cas12a/sgRNA-based nucleic acid detection platform provides an easy-to-use, quick, and cost-effective approach for identification of M. abscessus species and subspecies.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-019-03757-y