Structural analysis and potential anti-tumor activity of Sporisorium reilianum (Fries) polysaccharide

[Display omitted] •WM-NP-60 was isolated and purified from Sporisorium reilianum (Fries).•WM-NP-60 can inhibit cell proliferation in HepG2 and SGC7901 cells.•WM-NP-60 might be a potential therapeutic agent for the treatment of cancer. A neutral polysaccharide WM-NP-60 was successfully isolated and p...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2020-06, Vol.153, p.986-994
Main Authors: Kan, Lianbao, Chai, Yangyang, Li, Xiaoyan, Zhao, Min
Format: Article
Language:English
Subjects:
Anti-tumor activity
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Apoptosis - drug effects
Basidiomycota - chemistry
Fungal Polysaccharides - chemistry
Fungal Polysaccharides - pharmacology
G1 Phase Cell Cycle Checkpoints - drug effects
Hep G2 Cells
Humans
Methylation
Molecular Weight
Monosaccharides - analysis
Purification
Sporisorium reilianum polysaccharide
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] •WM-NP-60 was isolated and purified from Sporisorium reilianum (Fries).•WM-NP-60 can inhibit cell proliferation in HepG2 and SGC7901 cells.•WM-NP-60 might be a potential therapeutic agent for the treatment of cancer. A neutral polysaccharide WM-NP-60 was successfully isolated and purified from a phytopathogenic fungus Sporisorium reilianum (Fries). The characteristics and potential antitumor activities of WM-NP-60 were studied. WM-NP-60 was a water-soluble polysaccharide. The molecular weight of WM-NP-60 was 15.6 kDa. The main chain of WM-NP-60 was composed of β-1,6-D-Glcp and its side chains were β-1,3-D-Glcp. The side chains bound to the main chain with glycosyl groups at the C-3 positions. Gal might be attached to the backbone as a side chain or bound to the linear β-1,3-D-Glcp side chain. WM-NP-60 could inhibit the proliferation of HepG2 and SGC7901 cells in a dose-dependently manner. In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G1 phase and induced apoptosis of HepG2 and SGC7901 cells. Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2019.10.228