Performance of CDC Trioplex qPCR during a dengue outbreak in Brazil

•The Trioplex real-time RT-PCR assay was used in a geographic area with a current dengue outbreak and a low co-circulation of other arboviruses.•The multiplex method found that the test exhibits 95% sensitivity and 100% specificity.•These findings support the use of the Trioplex real-time RT-PCR ass...

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Bibliographic Details
Published in:Journal of clinical virology 2019-12, Vol.121, p.104208-104208, Article 104208
Main Authors: Colombo, Tatiana Elias, Versiani, Alice Freitas, Dutra, Karina Rocha, Rubiato, Julia Guimarães Dias, Galvão, Tayna Manfrin, Negri Reis, Andréia Francesli, Nogueira, Maurício Lacerda
Format: Article
Language:English
Subjects:
Dengue virus
Detection
Sensitivity
Specificity
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Summary:•The Trioplex real-time RT-PCR assay was used in a geographic area with a current dengue outbreak and a low co-circulation of other arboviruses.•The multiplex method found that the test exhibits 95% sensitivity and 100% specificity.•These findings support the use of the Trioplex real-time RT-PCR assay as a clinical screening test. In recent years real‑time reverse transcription polymerase chain reaction (real-time RT-PCR) has become a leading technique for nucleic acid detection and quantification of flaviviruses, including Dengue virus (DENV). Trioplex real-time RT-PCR has the advantages of providing the concurrent detection of Zika virus (ZIKV), DENV, and Chikungunya virus (CHIKV) RNA in human serum. This study sought to compare the sensitivity and specificity of the Trioplex real-time RT-PCR assay to those provided by CDC DENV TaqMan® RT-qPCR assay and conventional PCR when used for DENV detection in the context of a dengue epidemic. We analyzed 1656 serum samples from symptomatic patients with acute febrile disease for 5 days less between December 2018 and May 2019. The samples were tested using the various PCR-based assays. Of the 1656 serum samples analyzed, 713 (43%) were laboratory-confirmed as arboviruses: 99.86% (712/713) were confirmed as DENV and 0.14% (1/713) were confirmed as ZIKV. Next, 590 samples were selected, and of these, 331 samples (56.1%) were determined to be positive (Ct < 38) and 259 samples (43.9%) were determined to be negative (Ct > 38) using the Trioplex real-time RT-PCR assay. The multiplex method found that the test exhibits 95% sensitivity and 100% specificity. This evaluation demonstrates the capacity of the Trioplex real-time RT-PCR assay to detect DENV at a high sensitivity and specificity in a geographic area with a current dengue outbreak and a lower co-circulation of other arboviruses – such as ZIKV and CHIKV, and the results prove it´s applicability as clinical screening test that can serve as a confirmatory test.
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2019.104208