Sheathless Acoustic Fluorescence Activated Cell Sorting (aFACS) with High Cell Viability

In this work, we demonstrate a sheathless acoustic fluorescence activated cell sorting (aFACS) system by combining elasto-inertial cell focusing and highly focused traveling surface acoustic wave (FTSAW) to sort cells with high recovery rate, purity, and cell viability. The microfluidic sorting devi...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-12, Vol.91 (24), p.15425-15435
Main Authors: Li, Peixian, Liang, Minhui, Lu, Xiaoguang, Chow, Joycelyn Jia Ming, Ramachandra, Chrishan J. A, Ai, Ye
Format: Article
Language:English
Subjects:
Acoustics
Cardiomyocytes
Cell lines
Cell viability
Chemistry
Dilution
Flow cytometry
Fluorescence
Human influences
Interrogation
Microfluidics
Pluripotency
Purity
Recovery
Stem cells
Surface acoustic waves
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Summary:In this work, we demonstrate a sheathless acoustic fluorescence activated cell sorting (aFACS) system by combining elasto-inertial cell focusing and highly focused traveling surface acoustic wave (FTSAW) to sort cells with high recovery rate, purity, and cell viability. The microfluidic sorting device utilizes elasto-inertial particle focusing to align cells in a single file for improving sorting accuracy and efficiency without sample dilution. Our sorting device can effectively focus 1 μm particles which represents the general minimum size for a majority of cell sorting applications. Upon the fluorescence interrogation at the single cell level, individual cells are deflected to the target outlet by a ∼50 μm wide highly focused acoustic field. We have applied our aFACS to sort three different cell lines (i.e., MCF-7, MDA-231, and human-induced pluripotent stem-cell-derived cardiomyocytes; hiPSC-CMs) at ∼kHz with a sorting purity and recovery rate both of about 90%. A further comparison demonstrates that the cell viability drops by 35–45% using a commercial FACS machine, while the cell viability only drops by 3–4% using our aFACS system. The developed aFACS system provides a benchtop solution for rapid, highly accurate single cell level sorting with high cell viability, in particular for sensitive cell types.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b03021