Inhibitory Effect of 5-Aminoimidazole-4-Carbohydrazonamides Derivatives Against Candida spp. Biofilm on Nanohydroxyapatite Substrate

Candida can adhere and form biofilm on biomaterials commonly used in medical devices which is a key attribute that enhances its ability to cause infections in humans. Furthermore, biomaterial-related infections represent a major therapeutic challenge since Candida biofilms are implicated in antifung...

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Bibliographic Details
Published in:Mycopathologia (1975) 2019-12, Vol.184 (6), p.775-786
Main Authors: Gabriel, C., Grenho, L., Cerqueira, F., Medeiros, R., Dias, A. M., Ribeiro, A. I., Proença, M. F., Fernandes, M. H., Sousa, J. C., Monteiro, F. J., Ferraz, M. P.
Format: Article
Language:English
Subjects:
Antifungal agents
Antifungal Agents - pharmacology
Biocompatible Materials
Biofilms
Biofilms - drug effects
Biofilms - growth & development
Biological products
Biomedical and Life Sciences
Candida
Candida - drug effects
Candida - growth & development
Candida albicans
Candida albicans - drug effects
Candida albicans - growth & development
Cell Line - drug effects
Ceramic materials
Ceramics
Eukaryotic Microbiology
Health aspects
Humans
Imidazoles - pharmacology
Infection
Life Sciences
Medical equipment
Medical Microbiology
Microbial Ecology
Microbial Sensitivity Tests
Microbiology
Microscopy, Electron, Scanning
Original Article
Osteoblasts - drug effects
Physiological apparatus
Plant Sciences
Prostheses and Implants - microbiology
Prosthesis-Related Infections - drug therapy
Prosthesis-Related Infections - microbiology
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Summary:Candida can adhere and form biofilm on biomaterials commonly used in medical devices which is a key attribute that enhances its ability to cause infections in humans. Furthermore, biomaterial-related infections represent a major therapeutic challenge since Candida biofilms are implicated in antifungal therapies failure. The goals of the present work were to investigate the effect of three 5-aminoimidazole-4-carbohydrazonamides, namely ( Z )-5-amino-1-methyl- N′ -aryl-1 H -imidazole-4-carbohydrazonamides [aryl = phenyl (1a), 4-fluorophenyl (1b), 3-fluorophenyl (1c)], on Candida albicans and Candida krusei biofilm on nanohydroxyapatite substrate, a well-known bioactive ceramic material. To address these goals, both quantitative methods (by cultivable cell numbers) and qualitative evaluation (by scanning electron microscopy) were used. Compounds cytocompatibility towards osteoblast-like cells was also evaluated after 24 h of exposure, through resazurin assay. The three tested compounds displayed a strong inhibitory effect on biofilm development of both Candida species as potent in vitro activity against C. albicans sessile cells. Regarding cytocompatibility, a concentration-dependent effect was observed. Together, these findings indicated that the potent activity of imidazole derivatives on Candida spp. biofilms on nanohydroxyapatite substrate, in particular compound 1c, is worth further investigating. Graphic Abstract
ISSN:0301-486X
1573-0832
DOI:10.1007/s11046-019-00400-4