Complement system and phagocytosis in a colonial protochordate

In the present work, we investigated, in the colonial ascidian Botryllus schlosseri, the role of complement C3 (BsC3) in phagocytosis. We studied the modulation of BsC3 transcription in the course of the colonial blastogenetic cycle, with particular reference to the takeover, when apoptotic cells in...

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Published in:Developmental and comparative immunology 2020-02, Vol.103, p.103530-103530, Article 103530
Main Authors: Peronato, Anna, Drago, Laura, Rothbächer, Ute, Macor, Paolo, Ballarin, Loriano, Franchi, Nicola
Format: Article
Language:English
Subjects:
Activation
Antibodies
Apoptosis
Botryllus schlosseri
Colonial ascidians
Complement
Complement activation
Complement component C3
Gene expression
Gene knock-down
Hemocytes
Hybridization
Labeling
Phagocytes
Phagocytosis
Receptors
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Summary:In the present work, we investigated, in the colonial ascidian Botryllus schlosseri, the role of complement C3 (BsC3) in phagocytosis. We studied the modulation of BsC3 transcription in the course of the colonial blastogenetic cycle, with particular reference to the takeover, when apoptotic cells in the tissues of old zooids are cleared by circulating phagocytes. In situ hybridisation with BsC3 riboprobes labelled only morula cells, the most abundant haemocytes. Anti-hC3 antibody recognised morula cells and also phagocytes when haemocytes were previously incubated with zymosan. The inhibition of C3 activation prevented the labelling of phagocytes. In phagocytosis assays with haemocytes from colonies injected with anti-hC3 antibody or bsc3 iRNA, the capability to ingest target cells was significantly (p 
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2019.103530