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Microvirga calopogonii sp. nov., a novel alphaproteobacterium isolated from a root nodule of Calopogonium mucunoides in Southwest China
In this study, a Gram-negative, rod-shaped, and non-spore-forming bacterium, which was designated as strain CCBUA 65841 T , was isolated from a root nodule of Calopogonium mucunoides grown in Yunan Province of China. The sequence alignment results of 16S rRNA and four housekeeping genes (including g...
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Published in: | Antonie van Leeuwenhoek 2019-11, Vol.112 (11), p.1593-1602 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | In this study, a Gram-negative, rod-shaped, and non-spore-forming bacterium, which was designated as strain CCBUA 65841
T
, was isolated from a root nodule of
Calopogonium mucunoides
grown in Yunan Province of China. The sequence alignment results of 16S rRNA and four housekeeping genes (including
gyrB
,
recA
,
dnaK
and
rpoB
) indicated the isolated strain is a member of the genus
Microvirga
, closely related to
Microvirga lotononidis
WSM3557
T
. In addition, results of genome average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) had revealed the lower values (ANI ≤ 88.72%, dDDH ≤ 39.5%) between strain CCABU 65841
T
and other related
Microvirga
species. The genome of the novel strain exhibits a G + C content of 64.48% and contains 7296 protein-coding genes and 93 RNA genes. The major polar lipids were found to be phosphatidylcholine and phosphatidylethanolamine. The predominant cellar fatty acids were identified to be C16:0, C18:0, C19:0
cyclo
ω8c, summed feature 2, summed feature 3 and summed feature 8. Moreover, menaquinone 8 (MK-8) was detected to be the predominant quinone. Based on the phylogenetic and phenotypic dissimilarity, a novel species
Microvirga calopogonii
sp. nov. is proposed with the type strain CCABU 65841
T
(= LMG 25488
T
= HAMBI 3033
T
). |
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ISSN: | 0003-6072 1572-9699 |
DOI: | 10.1007/s10482-019-01285-5 |