Microvirga calopogonii sp. nov., a novel alphaproteobacterium isolated from a root nodule of Calopogonium mucunoides in Southwest China

In this study, a Gram-negative, rod-shaped, and non-spore-forming bacterium, which was designated as strain CCBUA 65841 T , was isolated from a root nodule of Calopogonium mucunoides grown in Yunan Province of China. The sequence alignment results of 16S rRNA and four housekeeping genes (including g...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2019-11, Vol.112 (11), p.1593-1602
Main Authors: Wang, Fang, Yang, Lei, Deng, Jia, Liu, Xiaoyun, Lu, Yanyuan, Chen, Wenxin, Wu, Jianrong
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Calopogonium mucunoides
China
Deoxyribonucleic acid
DNA
DnaK protein
Fabaceae - microbiology
Fatty acids
Genes
Genome, Bacterial
Genomes
Genomics - methods
Hybridization
Lecithin
Life Sciences
Lipids
Medical Microbiology
Menaquinones
Methylobacteriaceae - chemistry
Methylobacteriaceae - classification
Methylobacteriaceae - genetics
Methylobacteriaceae - isolation & purification
Microbiology
Microvirga
New species
Nucleotide sequence
Original Paper
Phenotype
Phosphatidylcholine
Phosphatidylethanolamine
Phylogeny
Plant Sciences
Quinones
RecA protein
RNA, Ribosomal, 16S - genetics
Root Nodules, Plant - microbiology
RpoB protein
rRNA 16S
Soil Microbiology
Soil Science & Conservation
Symbiosis
Whole Genome Sequencing
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Summary:In this study, a Gram-negative, rod-shaped, and non-spore-forming bacterium, which was designated as strain CCBUA 65841 T , was isolated from a root nodule of Calopogonium mucunoides grown in Yunan Province of China. The sequence alignment results of 16S rRNA and four housekeeping genes (including gyrB , recA , dnaK and rpoB ) indicated the isolated strain is a member of the genus Microvirga , closely related to Microvirga lotononidis WSM3557 T . In addition, results of genome average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) had revealed the lower values (ANI ≤ 88.72%, dDDH ≤ 39.5%) between strain CCABU 65841 T and other related Microvirga species. The genome of the novel strain exhibits a G + C content of 64.48% and contains 7296 protein-coding genes and 93 RNA genes. The major polar lipids were found to be phosphatidylcholine and phosphatidylethanolamine. The predominant cellar fatty acids were identified to be C16:0, C18:0, C19:0 cyclo ω8c, summed feature 2, summed feature 3 and summed feature 8. Moreover, menaquinone 8 (MK-8) was detected to be the predominant quinone. Based on the phylogenetic and phenotypic dissimilarity, a novel species Microvirga calopogonii sp. nov. is proposed with the type strain CCABU 65841 T (= LMG 25488 T  = HAMBI 3033 T ).
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-019-01285-5