Molecular Targeting Nanoprobes with Non-Overlap Emission in the Second Near-Infrared Window for in Vivo Two-Color Colocalization of Immune Cells

Monitoring specific immune cells in vivo will provide significant information for improving the therapeutic effect of immunotherapy. Herein, the in vivo two-color fluorescence molecular imaging of an important immune cell, myeloid-derived suppressor cell (MDSC), was realized by using quantum dot (QD...

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Published in:ACS nano 2019-11, Vol.13 (11), p.12830-12839
Main Authors: Yu, Guang-Tao, Luo, Meng-Yao, Li, Hao, Chen, Song, Huang, Biao, Sun, Zhi-Jun, Cui, Ran, Zhang, Mingxi
Format: Article
Language:English
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Summary:Monitoring specific immune cells in vivo will provide significant information for improving the therapeutic effect of immunotherapy. Herein, the in vivo two-color fluorescence molecular imaging of an important immune cell, myeloid-derived suppressor cell (MDSC), was realized by using quantum dot (QD)-based nanoprobes with non-overlap emission in the second near-infrared window (NIR-II, 1000–1700 nm). NIR-IIa and NIR-IIb QDs were conjugated with two MDSC-specific antibodies, respectively, and targeted the in vivo MDSCs together. Due to the suppressed photon scattering and diminished autofluorescence in the NIR-II window, the distribution of MDSCs in different organs and tissues was clearly revealed in a non-invasive way by the colocalization of two-color fluorescence from nanoprobes. The high-resolution imaging further confirmed the exact distribution of MDSCs in tumor immune microenvironment (TIME). Our results demonstrated that NIR-II fluorescence nanoprobes with molecular targeting ability provided a powerful tool for monitoring the dynamic change of immune cell populations in TIME in vivo, thus guiding the choice of clinical medicine and evaluating the therapeutic effect.
ISSN:1936-0851
1936-086X
DOI:10.1021/acsnano.9b05038