Oxidation of proline from the cyclin-binding motif in maize CDKA;1 results in lower affinity with its cyclin regulatory subunit

Cyclin dependent kinase A; 1 (CDKA; 1) is essential in G1/S transition of cell cycle and its oxidation has been implicated in cell cycle arrest during plant abiotic stress. In the present study, an evaluation at the molecular level was performed to find possible sites of protein oxidative modificati...

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Published in:Phytochemistry (Oxford) 2020-01, Vol.169, p.112165-112165, Article 112165
Main Authors: Méndez, Andrea A.E., Pena, Liliana B., Curto, Lucrecia M., Fernández, Marisa M., Malchiodi, Emilio L., Garza-Aguilar, Sara M., Vázquez-Ramos, Jorge M., Gallego, Susana M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Cyclin-Dependent Kinases - chemistry
Cyclin-Dependent Kinases - genetics
Cyclin-Dependent Kinases - metabolism
Cyclins - chemistry
Cyclins - metabolism
Maize cyclin dependent kinase A1
Models, Molecular
Oxidation-Reduction
Proline - chemistry
Proline - metabolism
Protein oxidation
Sequence Alignment
Zea mays - enzymology
Zea mays L
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Summary:Cyclin dependent kinase A; 1 (CDKA; 1) is essential in G1/S transition of cell cycle and its oxidation has been implicated in cell cycle arrest during plant abiotic stress. In the present study, an evaluation at the molecular level was performed to find possible sites of protein oxidative modifications. In vivo studies demonstrated that carbonylation of maize CDKA,1 is associated with a decrease in complex formation with maize cyclin D (CycD). Control and in vitro oxidized recombinant CDKA; 1 were sequenced by mass spectrometry. Proline at the PSTAIRE cyclin-binding motif was identified as the most susceptible oxidation site by comparative analysis of the resulted peptides. The specific interaction between CDKA; 1 and CycD6; 1, measured by surface plasmon resonance (SPR), demonstrated that the affinity and the kinetic of the interaction depended on the reduced-oxidized state of the CDKA; 1. CDKA; 1 protein oxidative modification would be in part responsible for affecting cell cycle progression, and thus producing plant growth inhibition under oxidative stress. [Display omitted] •Carbonylation of cyclin dependent kinase in vivo is associated with a decrease in complex formation with cyclin D (CycD).•CDKA; 1-CycD6; 1 interaction depends on the reduced -oxidized state of the CDKA; 1.•Proline from cyclin –binding motif of CDKA; 1 as a susceptible site for oxidation.
ISSN:0031-9422
1873-3700
DOI:10.1016/j.phytochem.2019.112165