Overproduction of lipoxygenase from Pseudomonas aeruginosa in Escherichia coli by auto-induction expression and its application in triphenylmethane dyes degradation

In this study, the bacterial lipoxygenase (LOX) gene from Pseudomonas aeruginosa ATCC27853 (pse-LOX) was cloned, sequenced and heterologous expressed in Escherichia coli by auto-induction expression strategy. Production of the recombinant pse-LOX (pse-rLOX) gene up to 23,850 U/mL (264 mg pure protei...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2020-03, Vol.129 (3), p.327-332
Main Authors: Lu, Jing, Zhang, Chong, Leong, Hui Yi, Show, Pau Loke, Lu, Fengxia, Lu, Zhaoxin
Format: Article
Language:English
Subjects:
Auto-induction expression
Biocatalyst
Decolorization
Escherichia coli
Pseudomonas aeruginosa
Recombinant lipoxygenase
Triphenylmethane dyes
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Summary:In this study, the bacterial lipoxygenase (LOX) gene from Pseudomonas aeruginosa ATCC27853 (pse-LOX) was cloned, sequenced and heterologous expressed in Escherichia coli by auto-induction expression strategy. Production of the recombinant pse-LOX (pse-rLOX) gene up to 23,850 U/mL (264 mg pure protein/L bacterial culture fluid) was observed in the end of this process. To the best of our knowledge, this is the first attempt to manipulate LOX heterologous expression process using auto-induction expression approach, and it is the highest production of recombinant LOX compared with other reports. Subsequently, the resulted pse-rLOX was proved to efficiently degrade triphenylmethane dyes such as malachite green, brilliant green and aniline blue. Generally, an overproduction of the LOX from P. aeruginosa was observed in E. coli, and this recombinant gene is a potential candidate as biocatalyst for triphenylmethane dyes decolorization.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2019.09.006