Characterization of intratumoral and circulating IL-10-producing B cells in gastric cancer

The tumor microenvironment harbors multiple immunosuppressive mechanisms, many of which involve suppressive immune cells. B regulatory (Breg) cells, as critical regulators of immune responses, were investigated in patients with gastric carcinoma. In the present study, the B cells that expressed IL-1...

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Bibliographic Details
Published in:Experimental cell research 2019-11, Vol.384 (2), p.111652-111652, Article 111652
Main Authors: Hu, Hong-Tao, Ai, Xiaoshun, Lu, Minxue, Song, Zongchang, Li, Hailiang
Format: Article
Language:English
Subjects:
B cell
Breg
CD27
Gastric cancer
IL-10
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Summary:The tumor microenvironment harbors multiple immunosuppressive mechanisms, many of which involve suppressive immune cells. B regulatory (Breg) cells, as critical regulators of immune responses, were investigated in patients with gastric carcinoma. In the present study, the B cells that expressed IL-10 were highly enriched in tumor-infiltrating B cells, and could also be found at reduced frequencies in circulating B cells. These cells expressed high CD19 and CD20, and were almost exclusively CD27+CD10−. The IL-10 expression was significantly higher in CD27+CD10--sorted B cells than in CD27−CD10--sorted B cells. In an in vitro coculture of B cells and autologous T cells, CD27+CD10− B cells were capable of reducing the levels of CD4 T cell-mediated IFNγ, TNF, and IL-17 expression and the levels of CD8 T cell-mediated IFNγ and TNF expression. These regulatory effects were dependent on IL-10 as well as CD80/CD86. Interestingly, CD27+CD10− B cells also significantly elevated IL-10 production from CD4 and CD8 T cells in an IL-10-dependent manner. Overall, we here report enrichment of IL-10-expressing CD27+CD10− B cells in the intratumoral environment, which could significantly alter the cytokine production profile by CD4 and CD8 T cells. •IL-10-expressing cells was highly enriched in tumor-infiltrating B cells.•IL-10-expressing cells were almost exclusively CD27+CD10−.•CD27+CD10− B cells suppressed expression of proinflammatory cytokines from T cells.•These regulatory effects were dependent on IL-10 as well as CD80/CD86.•CD27+CD10− B cells elevated IL-10 level from T cells in an IL-10-dependent manner.
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2019.111652