Redescription of Opalina triangulata (Heterokonta, Opalinea) from Fejervarya limnocharis based on morphological and molecular data

Opalinids are a large group of anaerobic protists, mainly inhabiting the cloacae of amphibians (frogs and toads). The classification of this group has not been fully resolved, because of a lack of molecular information. Here, we give a redescription of Opalina triangulata Metcalf, 1923, collected fr...

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Bibliographic Details
Published in:European journal of protistology 2019-10, Vol.71, p.125639-125639, Article 125639
Main Authors: Wang, Run-qiu, Zhao, Wei-shan, Hu, Guang-ran, Ponce-Gordo, Francisco, Zou, Hong, Li, Wen-xiang, Wu, Shan-gong, Wang, Gui-tang, Li, Ming
Format: Article
Language:English
Subjects:
Animals
Anura - parasitology
DNA, Ribosomal - genetics
Frogs
ITS genes
Morphology
Opalina
Phylogenetic analysis
Phylogeny
SSU rDNA
Stramenopiles - classification
Stramenopiles - cytology
Stramenopiles - genetics
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Summary:Opalinids are a large group of anaerobic protists, mainly inhabiting the cloacae of amphibians (frogs and toads). The classification of this group has not been fully resolved, because of a lack of molecular information. Here, we give a redescription of Opalina triangulata Metcalf, 1923, collected from the rectum of the frog Fejervarya limnocharis, based on detailed morphological and molecular data. Our phylogenetic analyses confirmed the monophyly of Opalinata. Within it, Opalinea were monophyletic with O. triangulata and O. undulata as well as two Protoopalina species grouping together. Karotomorpha and Proteromonas did not group together confirming the paraphyly of Proteromonadea. Meanwhile, the ITS2 secondary structural similarities as well as G-C content revealed greater similitudes between Opalina species and P. lacertae than with Blastocystis hominis, which is in accordance with their position as sister clades in the SSU rDNA-based phylogenies.
ISSN:0932-4739
1618-0429
DOI:10.1016/j.ejop.2019.125639