Dexmedetomidine inhibits the NF-κB pathway and NLRP3 inflammasome to attenuate papain-induced osteoarthritis in rats

Context: Dexmedetomidine (Dex) has been reported to have an anti-inflammatory effect. However, its role on osteoarthritis (OA) has not been explored. Objective: This study investigates the effect of Dex on OA rat model induced by papain. Materials and methods: The OA Wistar rat model was induced by...

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Bibliographic Details
Published in:Pharmaceutical biology 2019-01, Vol.57 (1), p.649-659
Main Authors: Cheng, Fang, Yan, Feng-Feng, Liu, Yue-Peng, Cong, Yan, Sun, Ke-Fu, He, Xue-Ming
Format: Article
Language:English
Subjects:
Animal models
Animals
Anti-Inflammatory Agents - pharmacology
Arthritis
articular cartilage
Cartilage
Cartilage (articular)
Cartilage diseases
Cartilage, Articular - drug effects
Caspase-1
Collagen (type II)
Collagenase 3
Dexmedetomidine - pharmacology
Disease
Drug dosages
Gait
Hospitals
IL-1β
Inflammasomes
Inflammasomes - drug effects
Inflammation
Inflammation - drug therapy
Injection
Interleukin 18
Interleukin 6
Joint pain
Knee
Laboratory animals
Male
Matrix Metalloproteinase 13
Models, Animal
NF-kappa B - metabolism
NF-κB protein
NLR Family, Pyrin Domain-Containing 3 Protein - metabolism
Osteoarthritis
Osteoarthritis - chemically induced
Osteoarthritis - drug therapy
Pain
Papain
Papain - pharmacology
paw withdrawal mechanical threshold
paw withdrawal thermal latency
Rats
Rats, Wistar
Rodents
Signal Transduction - drug effects
Tumor necrosis factor-α
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Summary:Context: Dexmedetomidine (Dex) has been reported to have an anti-inflammatory effect. However, its role on osteoarthritis (OA) has not been explored. Objective: This study investigates the effect of Dex on OA rat model induced by papain. Materials and methods: The OA Wistar rat model was induced by intraluminal injection of 20 mL of papain mixed solution (4% papain 0.2 mL mixed with 0.03 mol L −1 l-cysteine 0.1 mL) into the right knee joint. Two weeks after papain injection, OA rats were treated by intra-articular injection of Dex (5, 10, or 20 μg kg −1 ) into the right knee (once a day, continuously for 4 weeks). Articular cartilage tissue was obtained after Dex treatment was completed. Results: The gait behavior scores (2.83 ± 0.49), PWMT (15.2 ± 1.78) and PTWL (14.81 ± 0.92) in H-DEX group were higher than that of OA group, while Mankin score (5.5 ± 0.81) was decreased (p 
ISSN:1388-0209
1744-5116
DOI:10.1080/13880209.2019.1651874