Antioxidant compounds extracted from Diaporthe schini using supercritical CO2 plus cosolvent

Endophytic fungi have been highlight in the production of secondary metabolites with different bioactive properties, such as in the production of the antioxidant compounds. Therefore, the objective of this work was the extraction of the antioxidant compounds from the biomass of Diaporthe schini usin...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2020, Vol.43 (1), p.133-141
Main Authors: da Rosa, Barbara Vargas, Kuhn, Kátia Regina, Ugalde, Gustavo Andrade, Zabot, Giovani Leone, Kuhn, Raquel Cristine
Format: Article
Language:English
Subjects:
Antioxidants
Biomass
Biotechnology
Carbon dioxide
Chemical activity
Chemical composition
Chemical compounds
Chemistry
Chemistry and Materials Science
Diaporthe
Endophytes
Environmental Engineering/Biotechnology
Ethanol
Fermentation
Food Science
Fungi
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Metabolites
Organic chemistry
Research Paper
Secondary metabolites
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Summary:Endophytic fungi have been highlight in the production of secondary metabolites with different bioactive properties, such as in the production of the antioxidant compounds. Therefore, the objective of this work was the extraction of the antioxidant compounds from the biomass of Diaporthe schini using supercritical carbon dioxide (CO 2 ) without and with ethanol as cosolvent. The biomass was produced by submerged fermentation and the parameters evaluated in the extraction process were: pressure (150–250 bar), temperature (40–60 ºC) and cosolvent [biomass: cosolvent ratio, 1:0, 1:0.75 and 1:1.5 (w/v)]. Extraction yield, antioxidant activity and chemical composition of the extracts were determined. The highest extraction yield (3.24 wt.%) and the best antioxidant activity against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (96.62%) were obtained at 40 ºC, 250 bar and biomass:cosolvent ratio of 1:1.5 (w/v). The chemical compounds 1,4-diaza-2,5-dioxo-3-isobutyl bicyclo[4.3.0]nonane and benzeneethanol identified in GC/MS could be responsible for the antioxidant activity found in this study. Graphic abstract
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-019-02211-9