Discovery of a novel Mycobacterium asiaticum PRA-hsp65 pattern

Twenty-one pulmonary sputum samples from nine Brazilian patients were analyzed by the PRA-hsp65 method for identification of Mycobacterium species and the results were compared by sequencing. We reported a mutation at the position 381, that generates a suppression cutting site in the BstEII enzyme,...

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Bibliographic Details
Published in:Infection, genetics and evolution genetics and evolution, 2019-12, Vol.76, p.104040-104040, Article 104040
Main Authors: da Silva, William Marco Vicente, Duarte, Mayara Henrique, de Carvalho, Luciana Distásio, de Souza Caldas, Paulo Cesar, Campos, Carlos Eduardo Dias, Redner, Paulo, Ramos, Jesus Pais
Format: Article
Language:English
Subjects:
Brazil
DNA, Bacterial - genetics
Humans
Identification
M. asiaticum
Mycobacterium - classification
Mycobacterium - genetics
Mycobacterium - isolation & purification
Mycobacterium Infections - microbiology
Phylogeny
Point Mutation
Polymerase Chain Reaction - methods
PRA-hsp65
Sputum - microbiology
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Summary:Twenty-one pulmonary sputum samples from nine Brazilian patients were analyzed by the PRA-hsp65 method for identification of Mycobacterium species and the results were compared by sequencing. We reported a mutation at the position 381, that generates a suppression cutting site in the BstEII enzyme, thus leading to a new PRA-hsp65 pattern for M. asiaticum identification. •HaeIII showed obtained were considered the same ones as those of M. asiaticum type 1 (105/115)•The BstEII result obtained was different (441pb) from M. asiatium type 1•The hsp65 sequences obtained from strains analyzed in silico confirmed the results that PRA-hsp65 showed•This new pattern was not found in PRASITE database
ISSN:1567-1348
1567-7257
DOI:10.1016/j.meegid.2019.104040