Adventitious root cultures of Oplopanax elatus inhibit LPS-induced inflammation via suppressing MAPK and NF-κB signaling pathways

Bioreactor-cultured adventitious roots (ARs) of the endangered medicinal plant Oplopanax elatus Nakai is a novel alternative plant material. To utilize ARs in the product production, the present study investigated the anti-inflammatory effect of O. elatus ARs. In the in vivo experiment, lipopolysacc...

Full description

Saved in:
Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2019-10, Vol.55 (9), p.766-775
Main Authors: Tian, Wen, Piao, Xuan-Mei, Yin, Cheng-Ri, Jiang, Xiao-Long, Sun, Hao-Ding, An, Xiao-Li, Jiang, Jun, Lian, Mei-Lan
Format: Article
Language:English
Subjects:
Acetic acid
Alveoli
Animal Genetics and Genomics
Animals
Biomedical and Life Sciences
Bioreactors
Bronchus
c-Jun protein
Cell Biology
Cell Culture
Developmental Biology
Endangered plants
Ethyl acetate
Herbal medicine
Humans
In vivo methods and tests
Inflammation
Inflammation - chemically induced
Inflammation - drug therapy
Inflammation - genetics
Inflammation - pathology
Interleukin 6
Interleukins
JNK protein
Kinases
Life Sciences
Lipopolysaccharides
Lipopolysaccharides - toxicity
Lung diseases
Lungs
Macrophages
MAP kinase
Medicinal plants
Metastases
Mice
Mitogen-Activated Protein Kinase Kinases - genetics
NF-kappa B - genetics
NF-κB protein
Nitric oxide
Nitric-oxide synthase
Nuclear transport
Oplopanax
Oplopanax - chemistry
Peritoneum
Phosphorylation
Plant Extracts - chemistry
Plant Extracts - pharmacology
Plant Roots - chemistry
Pretreatment
Prostaglandin E2
Prostaglandin endoperoxide synthase
Proteins
Signal transduction
Signal Transduction - drug effects
Signaling
Stem Cells
Transcription factors
Translocation
Tumor necrosis factor-TNF
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bioreactor-cultured adventitious roots (ARs) of the endangered medicinal plant Oplopanax elatus Nakai is a novel alternative plant material. To utilize ARs in the product production, the present study investigated the anti-inflammatory effect of O. elatus ARs. In the in vivo experiment, lipopolysaccharide (LPS)-induced acute lung injury disease model was established and several inflammatory indexes were determined. For the LPS-stimulated mice, after pretreatment of AR crude extract (200 mg/kg), cell infiltration in lungs was decreased, the production of proinflammatory mediators, including nitric oxide (NO), tumor necrosis factor (TNF)-α, and interleukin (IL)-6, and 1β in the bronchoalveolar lavage fluid was evidently reduced, which indicated that O. elatus ARs had an anti-inflammatory effect. In the in vitro experiment, ethyl acetate (EtOAc) fractions (12.5, 25, and 50 μg/mL) were used to treat LPS-induced peritoneal macrophages (PMs) of mice. The production of NO, prostaglandin E 2 , TNF-α, IL-6, and IL-1β in LPS-stimulated PMs was obviously inhibited ( p  
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-019-00396-7