Early pregnancy diagnosis at 21 days post artificial insemination using corpus luteum vascular perfusion compared to corpus luteum diameter and/or echogenicity in Nelore heifers

The objective was to compare the use of corpus luteum (CL) vascular perfusion to CL diameter and/or echogenicity to diagnose pregnancy at 21 d after timed-AI. Ovaries of Nelore heifers were assessed using ultrasonography in B-mode and color Doppler simultaneously 21 d after timed-AI (n = 113). Objec...

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Bibliographic Details
Published in:Animal reproduction science 2019-10, Vol.209, p.106144-106144, Article 106144
Main Authors: Andrade, J.P.N., Andrade, F.S., Guerson, Y.B., Domingues, R.R., Gomez-León, V.E., Cunha, T.O., Jacob, J.C.F., Sales, J.N., Martins, J.P.N., Mello, M.R.B.
Format: Article
Language:English
Subjects:
Animals
Bovine
Cattle
Corpus luteum
Corpus Luteum - blood supply
Corpus Luteum - cytology
Corpus Luteum - diagnostic imaging
Early Diagnosis
Estrus Synchronization
Female
Insemination, Artificial - veterinary
Organ Size
Predictive Value of Tests
Pregnancy
Pregnancy diagnosis
Pregnancy Tests - methods
Pregnancy Tests - veterinary
Pregnancy, Animal
Regional Blood Flow
Sensitivity and Specificity
Time Factors
Ultrasonography - methods
Ultrasonography - veterinary
Ultrasound
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Summary:The objective was to compare the use of corpus luteum (CL) vascular perfusion to CL diameter and/or echogenicity to diagnose pregnancy at 21 d after timed-AI. Ovaries of Nelore heifers were assessed using ultrasonography in B-mode and color Doppler simultaneously 21 d after timed-AI (n = 113). Objective evaluations were performed using an image processing software to extract the number of colored pixels (ColorPix), diameter (mm) and echogenicity/mm² (EchoPix) of the CL. Subjective evaluations of the CL were performed by five evaluators using scores of estimated vascular perfusion area of color Doppler scan videos and estimated CL size and qualitative echogenicity of B-mode scan videos. The reference pregnancy diagnosis was performed 33 d after timed-AI using an ultrasonic device. Corpus luteum ColorPix, diameter and EchoPix were highly correlated (P 
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2019.106144