DNA Import into Plant Mitochondria: Complex Approach for in organello and in vivo Studies

Natural competence of mitochondria for DNA uptake has been known for the last 20 years. Until the present time, all studies of this process have been conducted exclusively in isolated mitochondria, as no system for investigation of the DNA transport into the mitochondria in intact cells has been ava...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Moscow) 2019-07, Vol.84 (7), p.817-828
Main Authors: Tarasenko, T. A., Tarasenko, V. I., Koulintchenko, M. V., Klimenko, E. S., Konstantinov, Yu. M.
Format: Article
Language:English
Subjects:
Arabidopsis - metabolism
Biochemistry
Biological Transport - genetics
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Brassica napus - metabolism
Cytoplasm
Deoxyribonucleic acid
DNA
DNA, Mitochondrial - metabolism
Genome, Mitochondrial
Genome, Plant
Imports
In Vitro Techniques - methods
In vivo methods and tests
Life Sciences
Microbiology
Mitochondria
Mitochondria - metabolism
Mitochondrial DNA
Mitochondrial Proteins - genetics
Plant Cells - metabolism
Plant mitochondria
Protoplasts
Protoplasts - metabolism
Real-Time Polymerase Chain Reaction - methods
Staining and Labeling
Substrates
Transport
Zea mays - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Natural competence of mitochondria for DNA uptake has been known for the last 20 years. Until the present time, all studies of this process have been conducted exclusively in isolated mitochondria, as no system for investigation of the DNA transport into the mitochondria in intact cells has been available. The objective of this work was to improve and standardize the existing approaches for investigating DNA import into plant mitochondria in an in organello system. A method for detecting the import of fluorescently labeled DNA substrates has been developed. Based on the features of DNA import into the mitochondria, we suggested an efficient method for the evaluation of the DNA import efficiency by quantitative PCR. We also developed and characterized the in vivo system that allows to detect DNA transport from the cytoplasm to the mitochondrial matrix in Arabidopsis thaliana protoplasts. A combination of the proposed techniques for studying the DNA uptake by plant mitochondria might be useful for elucidating whether the properties of the mitochondrial DNA import established in the in organello system are preserved in vivo .
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297919070113