Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish

Here, we report a novel non-enzymatic cell dissociation method, based on our finding that adherent cells dissociate rapidly from the polystyrene culture dish when incubated in an l- or d-arginine-containing solution. We also demonstrate the successful detachment of confluent NIH/3T3 cell monolayers...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2019-12, Vol.83 (12), p.2272-2275
Main Authors: Ikeda, Takeshi, Ichikawa, Kohei, Shigeto, Hajime, Ishida, Takenori, Hirota, Ryuichi, Funabashi, Hisakage, Kuroda, Akio
Format: Article
Language:English
Subjects:
Animals
Arginine
Arginine - pharmacology
Cell Culture Techniques - instrumentation
cell dissociation
cell sheet fabrication
Cell Survival
Mice
NIH 3T3 Cells
polystyrene culture dish
Polystyrenes
Solutions
tissue culture polystyrene
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Summary:Here, we report a novel non-enzymatic cell dissociation method, based on our finding that adherent cells dissociate rapidly from the polystyrene culture dish when incubated in an l- or d-arginine-containing solution. We also demonstrate the successful detachment of confluent NIH/3T3 cell monolayers from the culture dish as a cell sheet by the addition of an arginine solution.
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2019.1659716