Screening of oleaginous algal strains from Chlamydomonas reinhardtii mutant libraries via density gradient centrifugation

Microalgae have a high potential to be utilized as feedstock for biofuels because they have high growth rates and do not compromise food production. Commercialized algae‐based biofuel production relies on the development of strains with high lipid content. Based on the relatively low density of lipi...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2019-12, Vol.116 (12), p.3179-3188
Main Authors: Sung, Young Joon, Choi, Hong Il, Lee, Jeong Seop, Hong, Min Eui, Sim, Sang Jun
Format: Article
Language:English
Subjects:
Algae
Biofuels
Biomass
Cell density
cell screening system
Centrifugation
Chlamydomonas reinhardtii
Commercialization
Density
density gradient centrifugation
Fatty acids
Fluorescence
Food production
Fuels
Growth rate
high lipid content strain
Lipids
microalgae
Screening
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Summary:Microalgae have a high potential to be utilized as feedstock for biofuels because they have high growth rates and do not compromise food production. Commercialized algae‐based biofuel production relies on the development of strains with high lipid content. Based on the relatively low density of lipids compared to other cellular components, density gradient centrifugation was used to isolate high lipid content algal strains from Chlamydomonas reinhardtii mutant libraries. The correlation between cell density and lipid content was confirmed by analysis of Nile red fluorescence intensity, total lipids, and total fatty acid methyl ester content. A strain isolated by this screening method had 50% higher lipid content and 7% lower cell density than the parent wild‐type strain. Consequently, we demonstrated that screening of algal strains with low cell density via continuous density gradient centrifugation allows simple, rapid, and inexpensive screening for high lipid content strains. The density gradient centrifugation was applied to isolate high lipid content algal strains from Chlamydomonas reinhardtii mutant libraries based on the relatively low density of lipids. A mutant strain selected by this screening method had 50% higher lipid content and 7% lower cell density than the parent wild‐type strain. Consequently, the authors demonstrated that screening of algal strains with low cell density via continuous density gradient centrifugation allows simple, rapid, and inexpensive screening for oleaginous algal strains.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.27149