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Construction of restorer lines and molecular mapping for restorer gene of hau cytoplasmic male sterility in Brassica napus
Key message Successfully constructing restorer lines for the hau CMS line and molecular mapping of Rfh to a 94 kb candidate region on chromosome A03 in Brassica napus. Cytoplasmic male sterility is a general phenomenon in almost 200 species, and the interaction between chimeric genes in mitochondria...
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Published in: | Theoretical and applied genetics 2019-09, Vol.132 (9), p.2525-2539 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Key message
Successfully constructing restorer lines for the
hau
CMS line and molecular mapping of
Rfh
to a 94 kb candidate region on chromosome A03 in
Brassica napus.
Cytoplasmic male sterility is a general phenomenon in almost 200 species, and the interaction between chimeric genes in mitochondria and restorer genes in nucleus may be responsible for restoration of male fertility.
Orf288
has been identified as a CMS-associated gene in the
hau
CMS line of
Brassica napus
and
Brassica juncea;
however, the restorer lines/genes have not been found yet. We therefore have successfully constructed two restorer lines in
B. napus
by extensive testcrossing and have mapped a major restorer gene
Rfh
to a physical distance of 94 kb on chromosome A03 by whole-genome resequencing and molecular markers. We found that the restorer line is indeed restored to male fertility at histological level. Comparative genomics and collinearity analysis between close relatives revealed that rearrangements and recombination may have happened and thus caused the production of
Rfh
or components of the restoration of fertility complex. Meanwhile, nuclear backgrounds with multiple loci and temperature were related to the variation and instability of restoration of fertility in three different populations. Our study provides new sights into the coevolution between restorer genes and CMS-associated genes as well as the cultivation of superior hybrids via molecular breeding. |
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ISSN: | 0040-5752 1432-2242 |
DOI: | 10.1007/s00122-019-03368-3 |