Novel electrochemical sensing of galactose using GalOxNPs/CHIT modified pencil graphite electrode

For the construction of galactose biosensor, chitosan was electropolymerised onto the pencil graphite electrode. This chitosan modified pencil graphite electrode acts as good matrix for immobilization of enzyme nanoparticles of galactose oxidase. Development of this nanocomposite was further confirm...

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Bibliographic Details
Published in:Carbohydrate research 2019-09, Vol.483, p.107749-107749, Article 107749
Main Authors: Sharma, Mamta, Yadav, Pooja, Sharma, Minakshi
Format: Article
Language:English
Subjects:
Better
Biosensing Techniques - instrumentation
Biosensor
Chitosan
Chitosan - chemistry
Electrodes
Enzymes, Immobilized - chemistry
Galactose
Galactose - analysis
Galactose Oxidase - chemistry
Graphite
Limit of Detection
Microscopy, Electron, Scanning
Nanocomposites - chemistry
Nanoparticle
Spectroscopy, Fourier Transform Infrared
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Summary:For the construction of galactose biosensor, chitosan was electropolymerised onto the pencil graphite electrode. This chitosan modified pencil graphite electrode acts as good matrix for immobilization of enzyme nanoparticles of galactose oxidase. Development of this nanocomposite was further confirmed by Fourier transform infrared spectroscopy and scanning electron microscopy. The presence of chitosan makes the present galactose biosensor more efficient, reproducible and stable. The sensitivity was reported 7 × 10−3 mA/mM/cm2 with linear range from 0.05 to 25 mM and better detection limit of 0.05 mM. When the solution of galactose was spiked with 0.5 mM and 1 mM, the analytical recoveries were found 98.6% and 97.6%. A better storage stability was achieved (90days) when compared to earlier reported biosensors. Schematic representation of GalOxNPs/CHIT/PGE biosensor. [Display omitted] •Constructed an improved amperometric galactose biosensor based on GalOxNPs/CHIT) modified PGE.•Biosensor showed optimum current within 2 s, pH 6.5 and temperature 35 °C.•The limit of detection and working range of present biosensor were 0.05 mM and 0.05–25 mM respectively.•The enzyme electrode lost 40% of its initial activity following 90 days of its normal uses, when stored at 4 °C.•Biosensor measured galactose level in different samples of baby food products.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2019.107749