ClTI, a Kunitz trypsin inhibitor purified from Cassia leiandra Benth. seeds, exerts a candidicidal effect on Candida albicans by inducing oxidative stress and necrosis

Cassia leiandra is an Amazonian plant species that is used popularly for the treatment of mycoses. Recently, a protease inhibitor, named ClTI, with insecticidal activity against Aedes aegypti, was purified from the mature seeds of C. leiandra. In this work, we show that ClTI has antifungal activity...

Full description

Saved in:
Bibliographic Details
Published in:Biochimica et biophysica acta. Biomembranes 2019-11, Vol.1861 (11), p.183032-183032, Article 183032
Main Authors: Araújo, Nadine M.S., Dias, Lucas P., Costa, Helen P.S., Sousa, Daniele O.B., Vasconcelos, Ilka M., de Morais, Glaucia A., Oliveira, Jose T.A.
Format: Article
Language:English
Subjects:
Antifungal Agents - metabolism
Antifungal Agents - pharmacology
Aprotinin - metabolism
Aprotinin - pharmacology
Candida - drug effects
Candida albicans
Candida albicans - drug effects
Candidicidal
Cassia - metabolism
Cassia leiandra
Microbial Sensitivity Tests
Necrosis - metabolism
Oxidative Stress - drug effects
Seeds - metabolism
Trypsin
Trypsin inhibitor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cassia leiandra is an Amazonian plant species that is used popularly for the treatment of mycoses. Recently, a protease inhibitor, named ClTI, with insecticidal activity against Aedes aegypti, was purified from the mature seeds of C. leiandra. In this work, we show that ClTI has antifungal activity against Candida species and describe its mode of action towards Candida albicans. This study is relevant because the nosocomial infections caused by Candida species are a global public health problem that, together with the growing resistance to current drugs, has increased the urgency of the search for new antifungal compounds. ClTI inhibited the growth of Candida albicans, Candida tropicalis, Candida parapsilosis, and Candida krusei. However, ClTI was more potent against C. albicans. The candidicidal mode of action of ClTI on C. albicans involves enhanced cell permeabilization, alteration of the plasma membrane proton-pumping ATPase function (H+ -ATPase), induction of oxidative stress, and DNA damage. ClTI also exhibited antibiofilm activity and non-cytotoxicity to mammalian cells. These results indicate that ClTI is a promising candidate for the future development of a new, natural, and safe agent for the treatment of infections caused by C. albicans. [Display omitted] •ClTI is a kunitz trypsin inhibitor purified from Cassia leiandra Benth. seeds.•ClTI exerted anticandidicidal effect on Candida albicans.•ClTI induced ROS, damaged the cell surface, and caused necrosis in C. albicans cells.•ClTI has great potential as an antifungal drug against C. albicans.
ISSN:0005-2736
1879-2642
DOI:10.1016/j.bbamem.2019.183032