Characterization and role of PGK from Litopenaeus vannamei in WSSV infection

Phosphoglycerate kinase (EC 2.7.2.3, PGK) catalyses the reversible transfer of a phosphate group from 1,3-diphosphoglyceric acid and ADP to produce 3-phosphoglyceric acid and ATP, which represents the initial production of ATP during glycolysis; therefore, PGK is a key enzyme in the energy metabolis...

Full description

Saved in:
Bibliographic Details
Published in:Fish & shellfish immunology 2019-10, Vol.93, p.144-152
Main Authors: Li, Fei-Xiang, Zhang, Yong-Sheng, Yao, Cui-Luan
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Arthropod Proteins - chemistry
Arthropod Proteins - genetics
Arthropod Proteins - immunology
Base Sequence
Gene Expression Profiling
Gene Expression Regulation - immunology
Immune response
Immunity, Innate - genetics
Litopenaeus vannamei
Metabolism
Penaeidae - enzymology
Penaeidae - genetics
Penaeidae - immunology
PGK
Phosphoglycerate Kinase - chemistry
Phosphoglycerate Kinase - genetics
Phosphoglycerate Kinase - immunology
Phylogeny
RNAi
Sequence Alignment
White spot syndrome virus 1 - physiology
WSSV
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Phosphoglycerate kinase (EC 2.7.2.3, PGK) catalyses the reversible transfer of a phosphate group from 1,3-diphosphoglyceric acid and ADP to produce 3-phosphoglyceric acid and ATP, which represents the initial production of ATP during glycolysis; therefore, PGK is a key enzyme in the energy metabolism. To study the role of PGK in the resistance to WSSV infection in shrimp, the full-length cDNA of the PGK gene (LvPGK) from Litopenaeus vannamei was obtained by using homology cloning and RACE amplification. The tissue distribution of LvPGK and its expression changes in the main immune tissues after WSSV stimulation were obtained by quantitative real-time PCR. Furthermore, RNA interference (RNAi) was used to study the role of LvPGK in shrimp defending against WSSV infection. The results showed that the full-length cDNA sequence of LvPGK was 1855 bp, contained a 1248 bp open reading frame (ORF) encoding 415 amino acids, and included a conserved PGK domain. LvPGK presented ubiquitous expression in most examined tissues, with the most predominant expression in the muscle and the weakest expression in the intestine. LvPGK transcripts could be induced in the hemocytes and hepatopancreas by injection with WSSV. Both the replication of WSSV and the shrimp cumulative mortality decreased significantly after LvPGK knockdown (P 
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2019.07.048