Piceatannol alleviates inflammation and oxidative stress via modulation of the Nrf2/HO-1 and NF-κB pathways in diabetic cardiomyopathy

Diabetic cardiomyopathy (DCM) is one of the leading causes of morbidity and mortality in diabetic patients. Piceatannol (PIC) has protective effects against cardiovascular disease; however, it remains unknown whether it also protects against DCM. A Cell Counting Kit-8 (CCK-8) assay was used to evalu...

Full description

Saved in:
Bibliographic Details
Published in:Chemico-biological interactions 2019-09, Vol.310, p.108754-108754, Article 108754
Main Authors: Li, Hao, Shi, Youyang, Wang, Xuliang, Li, Ping, Zhang, Songyue, Wu, Tingting, Yan, Yaoyao, Zhan, Yi, Ren, Yue, Rong, Xing, Xia, Tianhe, Chu, Maoping, Wu, Rongzhou
Format: Article
Language:English
Subjects:
Cardiomyopathy
Diabetic
Inflammation
Nrf-2/HO-1
Oxidative stress
Piceatannol
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Diabetic cardiomyopathy (DCM) is one of the leading causes of morbidity and mortality in diabetic patients. Piceatannol (PIC) has protective effects against cardiovascular disease; however, it remains unknown whether it also protects against DCM. A Cell Counting Kit-8 (CCK-8) assay was used to evaluate the effects of PIC on the viability of high glucose (HG)-induced H9C2 cells. Protein expression and mRNA levels were detected by western blotting and real-time polymerase chain reaction (RT-PCR), respectively. In vivo, physical and biochemical analyses, together with transthoracic echocardiography and hemodynamic measurements, were used to detect the effects of PIC treatment on cardiac function in DCM rats. Reactive oxygen species production was determined using an ELISA kit, and inflammatory cytokines were detected by RT-PCR. Pathological changes were assessed by hematoxylin-eosin staining, immunohistochemical staining, and TUNEL staining. According to the results, PIC treatment improved cell viability and inhibited cell apoptosis in HG-induced H9C2 cardiac myoblasts. In addition, PIC not only attenuated the over-production of interleukin-6 (IL-6) (P 
ISSN:0009-2797
1872-7786
DOI:10.1016/j.cbi.2019.108754