Fabricating antimicrobial peptide-immobilized starch sponges for hemorrhage control and antibacterial treatment

[Display omitted] •Biocompatible macroporous sponges were developed from modified starch and HS-PEG-SH.•The sponges were covalently immobilized with antimicrobial peptide.•The sponges showed high fluid absorption capacity and well mechanical strength.•Antimicrobial peptide endowed sponges with effec...

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Bibliographic Details
Published in:Carbohydrate polymers 2019-10, Vol.222, p.115012-115012, Article 115012
Main Authors: Yang, Xiao, Liu, Wen, Xi, Guanghui, Wang, Mingshan, Liang, Bin, Shi, Yifen, Feng, Yakai, Ren, Xiangkui, Shi, Changcan
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - toxicity
Antimicrobial Cationic Peptides - chemistry
Antimicrobial Cationic Peptides - pharmacology
Antimicrobial Cationic Peptides - toxicity
Antimicrobial peptide
Biocompatibility
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Biocompatible Materials - toxicity
Escherichia coli - drug effects
Hemostatic sponge
Hemostatics - chemistry
Hemostatics - pharmacology
Hemostatics - toxicity
Immobilization
Immobilized Proteins - chemistry
Immobilized Proteins - pharmacology
Immobilized Proteins - toxicity
Male
Methicillin-Resistant Staphylococcus aureus - drug effects
Mice
Microbial Sensitivity Tests
Polyethylene Glycols - chemistry
Polyethylene Glycols - toxicity
Porosity
Rats, Sprague-Dawley
Solanum tuberosum - chemistry
Staphylococcus aureus - drug effects
Staphylococcus epidermidis - drug effects
Starch - chemistry
Starch - toxicity
Surgical Sponges
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Summary:[Display omitted] •Biocompatible macroporous sponges were developed from modified starch and HS-PEG-SH.•The sponges were covalently immobilized with antimicrobial peptide.•The sponges showed high fluid absorption capacity and well mechanical strength.•Antimicrobial peptide endowed sponges with effective and inherent antibacterial activity.•The sponges achieved high hemostasis and infection prevention simultaneously. It is important to control immediate hemorrhage and prevent infection simultaneously in the wound management. However, most of hemostatic materials are associated with low efficiency of hemostasis, poor biocompatibility and lack of antimicrobial properties. A kind of starch-based macroporous sponges (KR-Sps) immobilized covalently with antimicrobial peptide KR12 using highly efficient thiol-ene photo click reaction were developed. The physical properties of these sponges could be fine-tuned by varying the ratio of modified starch/HS-PEG-SH and the polymer concentration. The in vitro and vivo results demonstrated that KR-Sps induced thrombosis, shortened clotting time and reduced the blood loss at bleeding site. Besides, KR12 immobilized sponge exhibited inherent antimicrobial properties against Gram (+) and (−) bacteria and methicillin-resistant Staphylococcus aureus (MRSA), which could maintain at least 5 days. Therefore, KR-Sps were believed to be an excellent candidate as hemostatic and antimicrobial product for the intraoperative wound management.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2019.115012