Development of canine X-chromosome inactivation pattern analysis for the detection of cell clonality by incorporating the examination of the SLIT and NTRK-like family member 4 (SLITRK4) gene

X-chromosome inactivation pattern (XCIP) analysis can be used to assess the clonality of cell populations of various origin by distinguishing the methylated X chromosome from the unmethylated X chromosome. In this study, the utility of XCIP analysis was improved by incorporating the examination of A...

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Bibliographic Details
Published in:Research in veterinary science 2019-08, Vol.125, p.170-175
Main Authors: Tomita, A., Mochizuki, H., Tsuboi, M., Ogura, I., Igarashi, H., Goto-Koshino, Y., Takahashi, M., Ohmi, A., Tomiyasu, H., Ohno, K., Nakagawa, T., Uchida, K., Nishimura, R., Tsujimoto, H.
Format: Article
Language:English
Subjects:
Adrenal glands
Androgen receptor
Androgen receptors
Chromosomes
Clonality
Deactivation
Dog
Dogs
Heterozygosity
Inactivation
Pattern analysis
Polyglutamine
Population studies
Skin cancer
SLITRK4
Tissues
Trinucleotide repeats
Veterinary medicine
X chromosomes
X-chromosome inactivation
XCIP
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Summary:X-chromosome inactivation pattern (XCIP) analysis can be used to assess the clonality of cell populations of various origin by distinguishing the methylated X chromosome from the unmethylated X chromosome. In this study, the utility of XCIP analysis was improved by incorporating the examination of AC dinucleotide repeats in SLIT and NTRK-like family member 4 (SLITRK4) gene into the previously reported CAG repeat examination of androgen receptor (AR) gene in dogs. The rate of heterozygosity when both genes were analysed (125/150, 83.3%) was higher than AR gene examination alone (86/150, 57.3%). Blood samples from heterozygous dogs in either AC-1 or AC-2 of SLITRK4 gene were examined for the corrected inactivation allele ratio (CIAR), resulting in the determination of a reference range of CIAR 3.8, indicating the presence of a clonal population. Through the present study, the availability of canine XCIP analysis was improved by incorporating the examination of the SLITRK4 gene, providing a highly useful laboratory examination system for the detection of the clonality of various cell/tissue samples in dogs. •Availability of the canine XCIP analysis system was improved by examining both AR and SLITRK4 gene.•XCIP analysis developed here was available in 83% of female dogs.•Presence of clonal population was indicated by XCIP analysis in 51% of neoplastic tissue.•XCIP analysis can be used to detect clonal cell population and to help the diagnosis of neoplastic disease of various origin.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2019.06.004