Metabolomics analysis of Pulsatilla decoction on treatment of wetness‐heat‐induced diarrhea in rats based on UPLC–Q/TOF–MS/MS

Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness‐heat‐induced diarrhea (WHD). To investigate the therapeutic effects of PD in the treatment of WHD and elucidate the potential mechanism, we used a metabolomics strategy on th...

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Bibliographic Details
Published in:Biomedical chromatography 2019-11, Vol.33 (11), p.e4629-n/a
Main Authors: Hua, Yong‐li, Ma, Qi, Li, Wei, Zhang, Xiao‐song, Cheng, Xiao‐hua, Jia, Ya‐qian, Peng, Xiao‐ting, Yao, Wan‐ling, Ji, Peng, Hu, Jun‐jie, Wei, Yan‐ming
Format: Article
Language:English
Subjects:
Animals
Chromatography, High Pressure Liquid
Cytokines - blood
Diarrhea - etiology
Diarrhea - metabolism
Drugs, Chinese Herbal - pharmacology
Female
Hot Temperature - adverse effects
Male
Metabolome - drug effects
Metabolomics
Pulsatilla
Pulsatilla decoction
Rats
Rats, Wistar
Reproducibility of Results
Tandem Mass Spectrometry
UPLC–Q/TOF–MS/MS
wetness‐heat induced diarrhea
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Summary:Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness‐heat‐induced diarrhea (WHD). To investigate the therapeutic effects of PD in the treatment of WHD and elucidate the potential mechanism, we used a metabolomics strategy on the base of ultraperformance liquid chromatography coupled with quadrupole time‐of‐flight/mass spectrometry (UPLC–Q/TOF–MS/MS) and analyzed the serum samples of 32 rats to identify differential metabolites and pathways associated with the PD treatment of WHD. With variable importance for projection >1.0 in the Orthogonal partial least‐squares discriminant analysis (OPLS‐DA ) models and FC ≥1.2 or ≤0.8, 67 differential metabolites in the model and control groups and 33 differential metabolites in the model and PD groups were screened. A total of 23 differential metabolites were selected based on Venny analysis. Functional analysis showed that the differential metabolites identified were primarily involved in pentose and glucuronate interconversions, glycerophospholipid metabolism, tryptophan metabolism, starch and sucrose metabolism, and glycerolipid metabolism. This study suggested that PD exerts inhibitory effects on WHD. In particular, the significant roles of PD for treating WHD lie in regulating perturbed energy metabolism, glycerophospholipid metabolism and glycerolipid metabolism, and promoting lysoPC production restoring the function of intestinal tract.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.4629