Relationship between TNF-α, MMP-8, and MMP-9 levels in gingival crevicular fluid and the subgingival microbiota in periodontal disease

Periodontitis is modulated by a complex dysbiotic microbiota, these species stimulate upward the production of pro-inflammatory cytokines such as TNF-α, which, in turn, upregulates the production of bone resorption molecules. Enzymes such as MMP-8 and 9 have been associated with the destructive dise...

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Bibliographic Details
Published in:Odontology 2020-01, Vol.108 (1), p.25-33
Main Authors: Romero-Castro, Norma Samanta, Vázquez-Villamar, Mirna, Muñoz-Valle, José Francisco, Reyes-Fernández, Salvador, Serna-Radilla, Victor Othón, García-Arellano, Samuel, Castro-Alarcón, Natividad
Format: Article
Language:English
Subjects:
Bone loss
Bone resorption
Dentistry
Enzyme-linked immunosorbent assay
Gelatinase B
Gingiva
Gingival Crevicular Fluid
Gum disease
Humans
Hybridization
Inflammation
Matrix Metalloproteinase 8
Matrix Metalloproteinase 9
Medicine
Microbiota
Neutrophil collagenase
Oral and Maxillofacial Surgery
Original Article
Periodontal diseases
Periodontitis
Periodontium
Porphyromonas gingivalis
Prevotella intermedia
Species
Tumor Necrosis Factor-alpha
Tumor necrosis factor-α
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Summary:Periodontitis is modulated by a complex dysbiotic microbiota, these species stimulate upward the production of pro-inflammatory cytokines such as TNF-α, which, in turn, upregulates the production of bone resorption molecules. Enzymes such as MMP-8 and 9 have been associated with the destructive disease. This study evaluated the composition of periodontal microbiota with the checkerboard hybridization technique and its correlation with TNF-α, MMP-8, and MMP-9 evaluated with ELISA, of 80 patients (45 healthy, and 35 with chronic periodontitis). The frequency of the 18 species evaluated was higher in patients with bone loss compared with control group. TNF-α in gingival crevicular fluid was significantly higher in bone loss group ( p  
ISSN:1618-1247
1618-1255
DOI:10.1007/s10266-019-00435-5