Flaxseed Oil or n‐7 Fatty Acid‐Enhanced Fish Oil Supplementation Alters Fatty Acid Composition, Plasma Insulin and Serum Ceramide Concentrations, and Gene Expression in Lambs

The objective of this study was to examine the effects of flaxseed (FLAX) oil or 16‐carbon n‐7 fatty acid ‐enhanced fish oil (Provinal; POA) supplementation on serum, liver and skeletal muscle fatty acid concentrations, serum ceramide and plasma insulin concentrations, and gene expression. Lambs [n...

Full description

Saved in:
Bibliographic Details
Published in:Lipids 2019-06, Vol.54 (6-7), p.389-399
Main Authors: Duckett, Susan K., Furusho‐Garcia, Iraides, Rico, J. Eduardo, McFadden, Joseph W.
Format: Article
Language:English
Subjects:
Animals
Ceramide
Ceramides - blood
Dietary Supplements
Fatty acids
Fatty Acids - blood
Fish Oils - administration & dosage
Gene expression
Gene Expression Profiling
Insulin
Insulins - blood
Linseed Oil - administration & dosage
Palmitoleic acid
Polymerase Chain Reaction
RNA, Messenger - analysis
RNA, Messenger - genetics
Sheep - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of this study was to examine the effects of flaxseed (FLAX) oil or 16‐carbon n‐7 fatty acid ‐enhanced fish oil (Provinal; POA) supplementation on serum, liver and skeletal muscle fatty acid concentrations, serum ceramide and plasma insulin concentrations, and gene expression. Lambs [n = 18; 42 ± 5.6 kg body weight (BW); 7 months] were individually fed one of the three treatments: (1) control (CON), no oil supplement, (2) FLAX; at 0.1% of BW, or (3) POA at 0.1% of BW for 60 days. Daily feed intake and weight gain were decreased by 21% and 34%, respectively, for POA than FLAX. Liver and skeletal muscle concentrations of palmitoleic acid were greater by 396% and 87%, respectively, for POA than FLAX; whereas, liver and skeletal muscle α‐linolenic acid concentrations were greater by 199% and 118%, respectively, for FLAX. Supplementation with POA also had greater serum and tissue concentrations of eicosapentaenoic and docosahexaenoic acids. Serum glucose and plasma insulin concentrations were elevated with FLAX supplementation at the end of the study. Supplementation with POA altered serum ceramide concentrations compared to CON or FLAX. Oil supplementation, both FLAX and POA, downregulated expression of unesterified fatty acid receptors (FFAR) 1 and FFAR4 in the liver; however, oil supplementation upregulated expression of FFAR1 in muscle. Interleukin‐6 (IL6) and tumor necrosis factor‐α (TNFA) expression were downregulated with oil supplementation in the liver; however, FLAX upregulated TNFA in muscle. These results show that oil supplementation can enhance uptake and deposition of unique fatty acids that alter ceramide concentrations and gene expression in tissues.
ISSN:0024-4201
1558-9307
DOI:10.1002/lipd.12156