Gold nano-flowers (Au NFs) modified screen-printed carbon electrode electrochemical biosensor for label-free and quantitative detection of glycated hemoglobin

Glycated hemoglobin (HbA1c) represents the average glucose level over the past three months and has been considered as the most important biomarker for the diagnosis of Type Ⅱ diabetes (T2D). Herein, a label-free and quantitative electrochemical biosensor based on 4-mercaptophenylboronic acid (4-MPB...

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Bibliographic Details
Published in:Talanta (Oxford) 2019-08, Vol.201, p.119-125
Main Authors: Wang, Xiao, Su, Jing, Zeng, Dongdong, Liu, Gang, Liu, Lizhuang, Xu, Yi, Wang, Chenguang, Liu, Xinxin, Wang, Lu, Mi, Xianqiang
Format: Article
Language:English
Subjects:
Electrochemical biosensor
Glycated hemoglobin
Gold nano-flowers
Label-free and quantitative detection
Screen-printed carbon electrode
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Summary:Glycated hemoglobin (HbA1c) represents the average glucose level over the past three months and has been considered as the most important biomarker for the diagnosis of Type Ⅱ diabetes (T2D). Herein, a label-free and quantitative electrochemical biosensor based on 4-mercaptophenylboronic acid (4-MPBA) modified gold nano-flowers (Au NFs) substrate was developed for the determination of HbA1c. Under optimal conditions, the linear dynamic ranges of HbA1c (5 μg/mL - 1000 μg/mL) and HbA1c% (2%–20%) by cyclic voltammetry were achieved. The electrochemical biosensor showed great detection specificity towards HbA1c and relatively stability after storage at 4 °C. This method could also be applied in human serum system which holds great potential to be applied to monitor real blood samples of diabetes patients. In human serum system, the recovery rate could reach 103.8% and 99.0%. It could achieve fast detection, the total analysis time was less than 65 min, and the detection time was less than 10 s. Moreover, in terms of fabrication process, operation procedure, detection time and cost, this technique was superior to the current HbA1c detection methods suggesting great promise for the practical clinical use in the future. Schematic diagrams of (a) the 16-SPCE detection system and (b) the modification of 4-MPBA-Au NFs and the detection of HbA1c. The 16-channel screen-printed carbon electrode (16-SPCE) was firstly electrodeposited with gold nano-flowers (Au NFs), and then 4-mercaptobenzeneboronic acid (4-MPBA) was modified by Au-S bond. Boric acid can interact with HbA1c which can produce catalytic reduction of H2O2. [Display omitted] •We developed a 4-MPBA-Au NFs carbon nano-biosensing interface to improve the electron transfer and the response speed.•The label-free and quantitative determination of HbA1c was achieved by the 16-SPCE based on 4-MPBA-Au NFs substrate.•The wide dynamic range of HbA1c (5 μg/ml - 1000 μg/ml) and HbA1c% (2% - 20%) was obtained.•The novel HbA1c electrochemical biosensor could be applied in serum system with great specificity and long-term stability.•Comparing to the conventional method, our method was inexpensive, fast and simple.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2019.03.100