Increased expression of miR‐146a, miR‐10b, and miR‐21 in cancer stem‐like gastro‐spheres

Background Gastric cancer remains one of the leading causes of cancer‐associated mortalities globally. Accumulating evidence support the presence of gastric cancer stem cells (CSCs) and their role in the pathogenesis and therapeutic challenges of gastric cancer. MicroRNAs (miRNAs) may be influenced...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2019-10, Vol.120 (10), p.16589-16599
Main Authors: Bakhshi, Mahdieh, Asadi, Jahanbakhsh, Ebrahimi, Marzieh, Moradi, Abdol‐Vahab, Hajimoradi, Monireh
Format: Article
Language:English
Subjects:
AGS cell line
Biotechnology
Cancer
cancer stem‐like cell
Cell culture
Cisplatin
Gastric cancer
gastro‐sphere
Gene expression
microRNA
miRNA
MKN‐45 cell line
Pathogenesis
Polymerase chain reaction
Regulators
Reverse transcription
Spheres
Stem cells
Tumor cell lines
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Summary:Background Gastric cancer remains one of the leading causes of cancer‐associated mortalities globally. Accumulating evidence support the presence of gastric cancer stem cells (CSCs) and their role in the pathogenesis and therapeutic challenges of gastric cancer. MicroRNAs (miRNAs) may be influenced by the cellular differentiative state and as critical regulators of the cellular fate in development and cancer, can modulate the behavior of CSCs too. Here, we aimed to investigate the expression relevance of three prognostic miRNAs (miR‐21, miR‐10b, and miR‐146a) in CSCs of AGS and MKN‐45 gastric cancer cell lines. Methods Serial sphere‐forming assay in serum‐free culture medium was used to enrich the cellular population with stem‐like properties. Gastro‐spheres were characterized by evaluating the stemness gene expression, clonogenicity, and resistance to docetaxel and cisplatin in comparison with their parental cells. The expression level of miRNAs in gastro‐spheres and their parental cells was measured using quantitative reverse transcription polymerase chain reaction. Results Gastro‐spheres from both cell lines exhibit stem‐like properties: upregulated stemness associated genes (P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.28918