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Visualization of Intra‐neuronal Motor Protein Transport through Upconversion Microscopy
Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the moveme...
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Published in: | Angewandte Chemie International Edition 2019-07, Vol.58 (27), p.9262-9268 |
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Main Authors: | , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the movement of motor proteins in real‐time and in different depth planes. Herein, we present a dynamic imaging technique that fully exploits the characteristics of upconversion nanoparticles. This technique can be used as a microscopic probe for the quantitative in situ tracking of retrograde transport neurons with single‐particle resolution in multilayered cultures. This study may provide a powerful tool to reveal dynamic neuronal activity and intra‐axonal transport function as well as any associated neurodegenerative diseases resulting from mutation or impairment in the axonal transport machinery.
Monitoring motoring: The real‐time monitoring of the movement of motor proteins by conventional fluorescence microscopy has significant limitations owing to the low penetration depth of light and complex instrumentation. To address these issues, upconversion microscopy was used for the real‐time visualization of dynein tracking in axons and multi‐layered cell cultures. |
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ISSN: | 1433-7851 1521-3773 |
DOI: | 10.1002/anie.201904208 |