The effect of biogenic manufactured silver nanoparticles on human endothelial cells and zebrafish model

Human health and environment have been continuously getting exposure to toxic chemicals including nanomaterial; therefore, nontoxicity has recently attracted huge amount of attention. In this study, RU-AgNPs were synthesized by a green synthesis procedure and evaluated for their toxicity in human um...

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Published in:The Science of the total environment 2019-08, Vol.679, p.365-377
Main Authors: Khan, Imran, Bahuguna, Ashutosh, Krishnan, Manigandan, Shukla, Shruti, Lee, Hoomin, Min, Sang-Hyun, Choi, Dong Kyu, Cho, Youngjin, Bajpai, Vivek K., Huh, Yun Suk, Kang, Sun Chul
Format: Article
Language:English
Subjects:
Apoptosis
HUVECs
ROS
RU-AgNPs
Zebrafish
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Summary:Human health and environment have been continuously getting exposure to toxic chemicals including nanomaterial; therefore, nontoxicity has recently attracted huge amount of attention. In this study, RU-AgNPs were synthesized by a green synthesis procedure and evaluated for their toxicity in human umbilical vein endothelial cells (HUVECs) as well as on zebrafish embryos via apoptotic pathway. The synthesized RU-AgNPs were average in size (20–25 nm) with a negative surface charge of −13.43 mV. As a result, RU-AgNPs potentiated the formation of reactive oxygen species (ROS) in HUVECs as confirmed by the results of immunoblotting analysis using apoptotic markers, such as Bax, Bcl2, and cytochrome C. Moreover, the induction of apoptosis in HUVECs was also authenticated in a dose-dependent manner after the treatment with RU-AgNPs by the Incucyte analysis. In vivo trials conducted on zebrafish visualized the mortality, malformation, and imbalanced in the heart rate, and cell death of the whole embryo, including severe morphological changes in the yolk sac and the tail of zebrafish. Furthermore, the results of western blot analysis demonstrated the increasing intensity of apoptotic biomarkers such as Bax, Bcl2, and Cyto C, including enhanced production of ROS, validating the cell death in zebrafish larvae. In addition, chemically functionalized silver nanoparticles found to be more cytotoxic than biogenic functionalized silver nanoparticles. Above-mentioned findings clearly demonstrate that Ru-AgNPs cause the toxicity via ROS-induced apoptotic pathway. Therefore, it is necessary to decide RU-AgNPs toxicity levels before being used in any biomedical application. [Display omitted] •RU-AgNPs at certain level cause toxicity in HUVECs.•RU-AgNPs instigate cell senescence and apoptotic cell death in HUVECs.•RU-AgNPs can cause deformities in zebrafish larvae.•RU-AgNPs can induce oxidative stress and reduce heart rate.•RU-AgNPs promote cell senescence and apoptotic cell death in zebrafish larvae.
ISSN:0048-9697
1879-1026
DOI:10.1016/j.scitotenv.2019.05.045