Ovulatory follicular fluid induces sperm phagocytosis by neutrophils, but oviductal fluid around oestrus suppresses its inflammatory effect in the buffalo oviduct in vitro

We have recently shown that the conditioned media from bovine oviductal epithelial cell culture suppress sperm phagocytosis by neutrophils, suggesting that the oviduct around oestrus supplies the anti‐inflammatory microenvironment. To investigate the immune response of neutrophils toward the sperm a...

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Bibliographic Details
Published in:Molecular reproduction and development 2019-07, Vol.86 (7), p.835-846
Main Authors: Yousef, Mohamed S., Abd‐Elhafeez, Hanan H., Talukder, Anup K., Miyamoto, Akio
Format: Article
Language:English
Subjects:
Abattoirs
Animals
buffalo
Buffaloes - immunology
Cattle
Cell culture
Epithelial cells
Epithelial Cells - immunology
Epithelium
Estrus - physiology
Estrus cycle
Extracellular Traps - immunology
Fallopian Tubes - cytology
Fallopian Tubes - metabolism
Female
Fertilization
Fertilization - immunology
Follicular fluid
Follicular Fluid - immunology
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Immune response
In Vitro Techniques
Inflammation
Inflammation - immunology
Leukocytes (neutrophilic)
Male
Neutrophils
Neutrophils - immunology
Oviduct
Ovulation
Ovulation - immunology
Phagocytosis
Phagocytosis - immunology
Sperm
Spermatozoa - immunology
Transmission electron microscopy
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Summary:We have recently shown that the conditioned media from bovine oviductal epithelial cell culture suppress sperm phagocytosis by neutrophils, suggesting that the oviduct around oestrus supplies the anti‐inflammatory microenvironment. To investigate the immune response of neutrophils toward the sperm at ovulation in the buffalo oviduct, we examined (a) a detailed distribution of neutrophils in the oviduct in buffaloes, (b) the effect of ovulatory follicular fluid (FF) and oviductal fluid (OF) on sperm phagocytosis by neutrophils, and (c) the interaction of the ovulatory FF with OF on sperm phagocytosis by neutrophils in vitro. Buffalo oviducts were collected from healthy reproductive tracts at a local slaughterhouse. A detailed observation by histological examination and transmission electron microscopy revealed that neutrophils exist in the oviduct epithelium and lumen throughout the oestrous cycle in buffaloes. The number of neutrophils at the oestrus stage was higher in ampulla compared with those in isthmus, whereas they remained relatively constant at the dioestrus stage. Two hours of preincubation of neutrophils with FF enhanced sperm phagocytosis through the formation of neutrophil extracellular traps (NETs) together with H2O2 production, whereas OF around oestrus (eOF) suppressed sperm phagocytosis, NETs formation, and H2O2 production and relieved the above FF‐induced inflammatory response. Our findings show that neutrophils exist in the healthy cyclic oviduct across bovine species, and the OF supplies a strong anti‐inflammatory environment that could minimize the inflammatory effect of the FF that flows into the oviduct lumen after ovulation and supports the occurrence of fertilization. Neutrophils were identified in the buffalo oviductal lumen. Oviductal fluid at oestrus inhibits sperm phagocytosis by neutrophils via decreasing H2O2 production and suppression of NETs formation. It can overcome the temporary action of the follicular fluid after ovulation and support sperm against phagocytosis.
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.23164