Effects of Phase Polarity and Charge Balance Spinal Cord Stimulation on Behavior and Gene Expression in a Rat Model of Neuropathic Pain

Objective To investigate the effect of phase polarity and charge balance of spinal cord stimulation (SCS) waveforms on pain behavior and gene expression in a neuropathic pain rodent model. We hypothesized that differing waveforms will result in diverse behavioral and transcriptomics expression due t...

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Bibliographic Details
Published in:Neuromodulation (Malden, Mass.) Mass.), 2020-01, Vol.23 (1), p.26-35
Main Authors: Vallejo, Ricardo, Gupta, Ashim, Kelley, Courtney A., Vallejo, Alejandro, Rink, Jonathan, Williams, Joseph M., Cass, Cynthia L., Smith, William J., Benyamin, Ramsin, Cedeño, David L.
Format: Article
Language:English
Subjects:
Animals
Behavior
Chronic neuropathic pain
Chronic pain
Disease Models, Animal
Gene Expression
gene expression modulation
Male
Neuralgia
Neuralgia - genetics
Neuralgia - psychology
Neuralgia - therapy
Pain
Pain perception
Pain Threshold - psychology
phase polarity
Polarity
Polymerase chain reaction
Random Allocation
Rats
Rats, Sprague-Dawley
Spinal cord
spinal cord stimulation
Spinal Cord Stimulation - methods
Statistical analysis
Transcription
transcriptomics
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Summary:Objective To investigate the effect of phase polarity and charge balance of spinal cord stimulation (SCS) waveforms on pain behavior and gene expression in a neuropathic pain rodent model. We hypothesized that differing waveforms will result in diverse behavioral and transcriptomics expression due to unique mechanisms of action. Materials and Methods Rats were implanted with a four‐contact cylindrical mini‐lead and randomly assigned to two control (no‐pain and pain model) and five test groups featuring monophasic, as well as charge‐unbalanced and charge‐balanced biphasic SCS waveforms. Mechanical and cold allodynia were assessed to measure efficacy. The ipsilateral dorsal quadrant of spinal cord adjacent to the lead was harvested post‐stimulation and processed to determine gene expression via real‐time reverse‐transcriptase polymerase chain reaction (RT‐PCR). Gene expression, SCS intensity (mA), and behavioral score as percent of baseline (BSPB) were statistically analyzed and used to generate correlograms using R‐Studio. Statistical analysis was performed using SPSS22.0, and p 
ISSN:1094-7159
1525-1403
DOI:10.1111/ner.12964