Triacontanol-supported micropropagation of woody plants

The effectiveness of triacontanol in the micropropagation of two woody, economically important fruit plant species was investigated. Triacontanol was added to the routine multiplication and rooting media of apple (Malus domestica cv. JTE-E4) and sour cherry (Cerasus fruticosa cv. Probocskai) rootsto...

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Bibliographic Details
Published in:Plant cell reports 2001-01, Vol.20 (1), p.16-21
Main Authors: TANTOS, A, MESZAROS, A, FARKAS, T, SZALAI, J, HORVATH, G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Cerasus fruticosa
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
In vitro propagation: entire plant regeneration from tissues and cell cultures
Malus domestica
Methods. Procedures. Technologies
Plant cells and fungal cells
triacontanol
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Summary:The effectiveness of triacontanol in the micropropagation of two woody, economically important fruit plant species was investigated. Triacontanol was added to the routine multiplication and rooting media of apple (Malus domestica cv. JTE-E4) and sour cherry (Cerasus fruticosa cv. Probocskai) rootstocks at concentrations of 2, 5, 10 and 20 μg/l. It was found to increase the number of shoots and the fresh weight of apple in the multiplication phase and to enhance root number and chlorophyll content in the rooting phase. The addition of indole-3-butyric acid (IBA) to the media further improved the effect of triacontanol. A less pronounced effect could be seen in the multiplication phase of sour cherry, although there was an enhancement of shoot proliferation. In the rooting phase, however, the application of triacontanol caused a significant increase in the number of roots per plant, and this effect was further improved when triacontanol was combined with 0.5 mg indole-3-butyric acid/l.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990000282