Adventitious shoot regeneration from root, internode, petiole and leaf explants of Piper colubrinum Link

A single-step, high-frequency regeneration protocol has been standardised for Phytophthora-resistant wild pepper, Piper colubrinum (Link) using root, internode, leaf and petiole explants derived from in vitro plantlets. The effect of BA on shoot-bud induction and elongation was assessed by supplemen...

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Bibliographic Details
Published in:Plant cell reports 1998-06, Vol.17 (9), p.721-725
Main Authors: Kelkar, S.M, Krishnamurthy, K.V
Format: Article
Language:English
Subjects:
2,4-D
benzyladenine
Biological and medical sciences
Biotechnology
culture media
dose response
Eukaryotic cell cultures
explants
Fundamental and applied biological sciences. Psychology
in vitro culture
In vitro propagation: entire plant regeneration from tissues and cell cultures
induction medium
internodes
leaves
methodology
Methods. Procedures. Technologies
micropropagation
murashige and skoog medium
naphthaleneacetic acid
petioles
Phytophthora
Piper colubrinum
Plant cells and fungal cells
regenerative ability
roots
shoots
subculture medium
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Summary:A single-step, high-frequency regeneration protocol has been standardised for Phytophthora-resistant wild pepper, Piper colubrinum (Link) using root, internode, leaf and petiole explants derived from in vitro plantlets. The effect of BA on shoot-bud induction and elongation was assessed by supplementing half-strength MS medium (macronutrients at half the concentration) with different concentrations of BA, i.e. 0.2-10 mg l(-1) in induction media and 0, 0.2 and 0.5 mg l(-1) in subculture media. The interaction between culture period and BA concentration was studied by culturing the explants for 8, 15 and 30 days before the first subculture. The elongated shoots were rooted directly in soil and hardened in the greenhouse. The developed protocol would be useful in marker-assisted asymmetric hybridisation programmes involving wildtype Piper colubrinum and the cultivated species P. nigrum.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050472