Osa‐miR167d facilitates infection of Magnaporthe oryzae in rice

MicroRNAs (miRNAs) play important roles in rice response to Magnaporthe oryzae, the causative agent of rice blast disease. Studying the roles of rice miRNAs is of great significance for the disease control. Osa‐miR167d belongs to a conserved miRNA family targeting auxin responsive factor (ARF) genes...

Full description

Saved in:
Bibliographic Details
Published in:Journal of integrative plant biology 2020-05, Vol.62 (5), p.702-715
Main Authors: Zhao, Zhi‐Xue, Feng, Qin, Cao, Xiao‐Long, Zhu, Yong, Wang, He, Chandran, Viswanathan, Fan, Jing, Zhao, Ji‐Qun, Pu, Mei, Li, Yan, Wang, Wen‐Ming
Format: Article
Language:English
Subjects:
Disease control
Disease Resistance
Fungi
Gene expression
Gene Expression Regulation, Plant
Immunity
Infections
Inoculation
Magnaporthe oryzae
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
Mimicry
miRNA
Oryza - genetics
Oryza - metabolism
Oryza - microbiology
Plant Diseases - genetics
Plant Diseases - microbiology
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
Plants, Genetically Modified - microbiology
Rice blast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:MicroRNAs (miRNAs) play important roles in rice response to Magnaporthe oryzae, the causative agent of rice blast disease. Studying the roles of rice miRNAs is of great significance for the disease control. Osa‐miR167d belongs to a conserved miRNA family targeting auxin responsive factor (ARF) genes that act in developmental and stress‐induced responses. Here, we show that Osa‐miR167d plays a negative role in rice immunity against M. oryzae by suppressing its target gene. The expression of Osa‐miR167d was significantly suppressed in a resistant accession at and after 24 h post inoculation (hpi), however, its expression was significantly increased at 24 hpi in the susceptible accession upon M. oryzae infection. Transgenic rice lines over‐expressing Osa‐miR167d were highly susceptible to multiple blast fungal strains. By contrast, transgenic lines expressing a target mimicry to block Osa‐miR167d enhanced resistance to rice blast disease. In addition, knocking out the target gene ARF12 led to hyper‐susceptibility to multiple blast fungal strains. Taken together, our results indicate that Osa‐miR167d negatively regulate rice immunity to facilitate the infection of M. oryzae by downregulating ARF12. Thus, Osa‐miR167d‐ARF12 regulatory module could be valuable in improvement of blast‐disease resistance. Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases. Here we demonstrated that rice Osa‐miR167d facilitates the infection of M. oryzae by suppressing ARF12, and ARF12 is required for resistance to the blast disease. Our findings suggest that the Osa‐miR167d‐ARF12 regulatory module is valuable in blast‐disease breeding programs.
ISSN:1672-9072
1744-7909
DOI:10.1111/jipb.12816