Viromes in Xylariaceae fungi infecting avocado in Spain

Four isolates of Entoleuca sp., family Xylariaceae, Ascomycota, recovered from avocado rhizosphere in Spain were analyzed for mycoviruses presence. For that, the dsRNAs from the mycelia were extracted and subjected to metagenomics analysis that revealed the presence of eleven viruses putatively belo...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2019-06, Vol.532, p.11-21
Main Authors: Velasco, Leonardo, Arjona-Girona, Isabel, Cretazzo, Enrico, López-Herrera, Carlos
Format: Article
Language:English
Subjects:
Endornavirus
Entoleuca
Gammaflexivirus
Horizontal virus transmission
Multiple virus infections
Mycoviruses
Negative single-stranded RNA virus
Ourmia-like virus
Rosellinia necatrix
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Summary:Four isolates of Entoleuca sp., family Xylariaceae, Ascomycota, recovered from avocado rhizosphere in Spain were analyzed for mycoviruses presence. For that, the dsRNAs from the mycelia were extracted and subjected to metagenomics analysis that revealed the presence of eleven viruses putatively belonging to families Partitiviridae, Hypoviridae, Megabirnaviridae, and orders Tymovirales and Bunyavirales, in addition to one ourmia-like virus plus other two unclassified virus species. Moreover, a sequence with 98% nucleotide identity to plant endornavirus Phaseolus vulgaris alphaendornavirus 1 has been identified in the Entoleuca sp. isolates. Concerning the virome composition, the four isolates only differed in the presence of the bunyavirus and the ourmia-like virus, while all other viruses showed common patterns. Specific primers allowed the detection by RT-PCR of these viruses in a collection of Entoleuca sp. and Rosellinia necatrix isolates obtained from roots of avocado trees. Results indicate that intra- and interspecies horizontal virus transmission occur frequently in this pathosystem.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2019.03.021