Chemical-Modified Nucleotide-Based Elemental Tags for High-Sensitive Immunoassay

Multiplex biomolecular analysis with inductively coupled plasma mass spectrometry (ICP-MS) becomes increasingly important in clinical diagnosis and single cell analysis. However, the sensitivity of ICP-MS-based immunoassay is only comparable or lower than those of fluorescence methods at the present...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-05, Vol.91 (9), p.5980-5986
Main Authors: Hu, Zhian, Sun, Gongwei, Jiang, Wencan, Xu, Fujian, Zhang, Yuqing, Xia, Mengchan, Pan, Xingyu, Xing, Zhi, Zhang, Sichun, Zhang, Xinrong
Format: Article
Language:English
Subjects:
Alkynes
Atomic properties
Biocompatibility
Chains
Chemical synthesis
Chemistry
Cytometry
Deoxyribonucleic acid
Diagnosis
DNA
DNA biosynthesis
Fluorescence
Immunoassay
Inductively coupled plasma mass spectrometry
Mass spectrometry
Mass spectroscopy
Metal ions
Metals
Organic chemistry
Rare earth elements
Sensitivity
Sensitivity analysis
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Summary:Multiplex biomolecular analysis with inductively coupled plasma mass spectrometry (ICP-MS) becomes increasingly important in clinical diagnosis and single cell analysis. However, the sensitivity of ICP-MS-based immunoassay is only comparable or lower than those of fluorescence methods at the present stage. Therefore, designing elemental tags with a large number of metal atoms is necessary to achieve high-sensitive detection. In this work, we proposed a new strategy to build up elemental tag loading with hundreds of rare earth ions by coupling alkyne-DNA chains with rare earth element (REE)-DOTA complexes a click chemistry reaction. There are about 2 orders of magnitude improvement in sensitivity compared with single metal-ion tags. DNA chains with multialkynyl groups were facilely prepared by PCR synthesis. Moreover, the DNA-based elemental tags own excellent water-solubility and biocompatibility. The tags would be potentially applied to mass cytometry and clinical diagnosis.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b00405